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Selenium-addicted microbe Wautersiella enshiensis YLK-1 and application thereof

A technology for microorganisms and inorganic selenium, applied to selenium-loving microorganisms and their application fields, can solve the problems of unidentified microbial species and characteristics, etc.

Active Publication Date: 2015-12-09
SUZHOU SETEK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the invention patent did not isolate and purify related selenium-tolerant microorganisms, nor did it identify the species and characteristics of the microorganisms

Method used

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  • Selenium-addicted microbe Wautersiella enshiensis YLK-1 and application thereof
  • Selenium-addicted microbe Wautersiella enshiensis YLK-1 and application thereof
  • Selenium-addicted microbe Wautersiella enshiensis YLK-1 and application thereof

Examples

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Effect test

Embodiment 1

[0052] The application of the microorganism of the present invention in synthesizing organic selenium.

[0053] The microorganism YLX-1 strain of the present invention was inoculated into a TSB medium containing 1.5 mg / L of selenium (sodium selenite), cultured at 200 r / min at room temperature for 48 hours, and the cells and supernatant were collected by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacterial cells, respectively. The results showed that almost all of the Se in the supernatant was Se. 4+ , and most of the cells are selenocystine (SeCys 2)(table 5). This shows that the YLX-1 strain can reduce the inorganic selenium (Se) in the culture solution under the condition of 1.5mgSe / L 4+ ) into organic selenium (SeCys 2 ) and accumulated in the bacteria. Further collection of bacterial cells can be used as organic selenium addition in animal feed, etc....

Embodiment 2

[0058] The application of the microorganism of the present invention in synthesizing organic selenium.

[0059] The microorganism YLX-1 strain of the present invention was inoculated into a TSB culture solution containing 10 mg / L of selenium (sodium selenite), cultured at 200 r / min at room temperature for 72 hours, and the cells and supernatant were collected by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacterial cells, respectively. The results showed that almost all of the Se in the supernatant was Se. 4+ , and most of the cells are selenocystine (SeCys 2 ), a small amount of methylselenocysteine ​​(SeMeCys) and Se 4+ (Table 6). This shows that the YLX-1 strain can reduce the inorganic selenium (Se) in the culture solution under the condition of 10mgSe / L 4+ ) into organic selenium (SeCys 2 , SeMeCys), and accumulated in the bacteria. Further collecti...

Embodiment 3

[0065] The application of the microorganism of the present invention in the synthesis of nanometer selenium.

[0066] The microorganism YLX-1 strain of the present invention was inoculated into a TSB culture solution containing 100 mg / L of selenium (sodium selenite), and cultivated at 200 r / min at room temperature for 24 hours, and the solution was obviously red and turbid. After culturing for 48 hours, the cells and supernatant were collected by centrifugation, and it was found that the collected precipitate was a red suspension. Then, liquid chromatography-atomic fluorescence spectrometer (LC-GA-AFS) and atomic fluorescence spectrometer were used to identify the red suspension as almost elemental selenium by differential subtraction. Using scanning electron microscope combined with energy spectrum scattering analysis (SEM-EDS), the results showed that there were a large number of bacteria in the red suspension, and a large number of spherical objects with a size of 100-200nm...

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Abstract

The invention discloses selenium-addicted microbe Wautersiella enshiensis YLK-1 separated from a microbial mat sediment in a percolating water system in the selenium mining area of Enshi, Hubei, China and application of selenium-addicted microbe Wautersiella enshiensis YLK-1. The selenium-addicted microbe Wautersiella enshiensis YLK-1 is preserved in CCTCC (China Center for Type Culture Collection) with the preservation number of CCTCC No: M2013671. The selenium-addicted microbe Wautersiella enshiensis YLK-1 has the characteristics of selenium-addicted microbes and has a potential application value in microbial organic selenium conversion, microbial nano-selenium synthesis, selenium-containing ore microbial activation, selenium-rich microbial fertilizer preparation and selenium-contaminated environment remediation.

Description

technical field [0001] The invention relates to a selenium-loving microorganism and application thereof, in particular to a selenium-loving microorganism Wautersiellaenshiensis YLX-1 and its use in microbial transformation and synthesis of organic selenium, microbial synthesis of nano-selenium, microbial activation of selenium ore, selenium-enriched microbial fertilizer preparation and selenium pollution in the environment The application in restoration belongs to the field of biotechnology. Background technique [0002] Extreme microorganisms refer to microbial species suitable for living in extreme environments, including thermophilic bacteria, halophilic bacteria, alkalophilic bacteria, acidophilic bacteria, barophilic bacteria, psychrophilic bacteria, as well as radiation-resistant, drying-resistant and high-concentration metal-resistant bacteria. Ionic and extremely anaerobic microorganisms (Rothschild & Mancinelli, 2001; Chen Jun et al., 2006), the current research on ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12P3/00C05F17/00C02F3/34C12R1/01C02F101/10
CPCC02F3/34C05F17/00C12N1/20C12P1/04C12P3/00C12N1/205C12R2001/01Y02W30/40
Inventor 袁林喜
Owner SUZHOU SETEK
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