microbacterium oxydans YLX-2 and its application

A technology of microbacterium oxidizing and YLX-2, which is applied in the direction of bacteria, microorganisms, chemical instruments and methods, and can solve the problems of unidentified microorganism species and characteristics

Active Publication Date: 2019-02-12
安徽硒谷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the invention patent did not isolate and purify related selenium-tolerant microorganisms, nor did it identify the species and characteristics of the microorganisms

Method used

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  • microbacterium oxydans YLX-2 and its application
  • microbacterium oxydans YLX-2 and its application
  • microbacterium oxydans YLX-2 and its application

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Effect test

Embodiment 1

[0042] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0043] Inoculate the microorganism YLX-2 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 0.5mg / L, cultivate it at 200r / min for 48 hours at normal temperature, and collect the thalline and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the bacteria are selenocystine (SeCys 2 )(Table 4). This shows that YLX-2 bacterial strain can under the condition of 0.5mgSe / L, the inorganic selenium (Se 4+ ) into organic selenium (SeCys 2 ) and accumulate in the bacteria. Further collection of thalline can be used as organic selenium addition of animal feed etc., also can do further selenoamino ...

Embodiment 2

[0048] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0049] Inoculate the microorganism YLX-2 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 5 mg / L, cultivate it at 200 r / min for 72 hours at normal temperature, and collect the bacteria and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the bacteria are selenocystine (SeCys 2 ), a small amount of methylselenocysteine ​​(SeMeCys) and Se 4+ (table 5). This shows that YLX-2 bacterial strain can under 5mgSe / L condition, the inorganic selenium (Se 4+ ) into organic selenium (SeCys 2 and SeMeCys), and accumulated into the bacteria. Further collection of thalline can be used as organic s...

Embodiment 3

[0054] The application of the microorganism described in the invention in the synthesis of nanometer selenium.

[0055]The microorganism YLX-2 bacterial strain of the present invention is inoculated in the TSB culture fluid that contains selenium (sodium selenite configuration) 100mg / L, cultivates 24 hours at 200r / min under normal temperature, and the solution appears obvious red, turbid. After culturing for 48 hours, the cells and supernatant were collected by centrifugation, and it was found that the collected precipitate was a red suspension. Then, using liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) and atomic fluorescence spectrometer, it was identified that the composition of the red suspension was almost elemental selenium by subtraction method. The nano-selenium synthesized by the microorganism can be used as a source of selenium supplementation for selenium-deficient people, and can also be used as a selenium fertilizer or an animal feed additive. ...

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Abstract

The invention discloses microbacterium oxydans YLX-2 separated from a microbial mat sediment in a percolating water system in the selenium mining area of Enshi, Hubei, China and application of microbacterium oxydans YLX-2. The microbacterium oxydans YLX-2 is preserved in CCTCC (China Center for Type Culture Collection) with the preservation number of CCTCC No: M2013672. The microbacterium oxydans YLX-2 has the characteristics of selenium-addicted microbes and has a potential application value in microbial organic selenium conversion, microbial nano-selenium synthesis, selenium-containing ore microbial activation, selenium-rich microbial fertilizer preparation and selenium-contaminated environment remediation.

Description

technical field [0001] The present invention relates to a selenophilic microorganism and its application, in particular to a strain of Microbacterium oxydans YLX-2 and its use in microbial transformation and synthesis of organic selenium, microbial synthesis of nano-selenium, microbial activation of selenium ore, preparation of selenium-rich microbial fertilizers and selenium pollution The application in environmental restoration belongs to the field of biotechnology. Background technique [0002] Extremophiles refer to microbial species suitable for living in extreme environments, including thermophilic bacteria, halophilic bacteria, alkaliphilic bacteria, acidophilic bacteria, barophilic bacteria, psychrophilic bacteria, and radiation-resistant, dry-resistant, and high-concentration metal-resistant bacteria. Ion and extreme anaerobic microorganisms (Rothschild&Mancinelli, 2001; Chen Jun et al., 2006). At present, the research on extremophiles is mainly in areas such as mid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P3/00C22B3/18C05F11/08C02F3/34C12R1/01
CPCY02P10/20
Inventor 袁林喜
Owner 安徽硒谷生物科技有限公司
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