Characteristic protein for identifying Chinese date honey flower source

A technology of jujube nectar and protein, applied in the field of food science, can solve the problems of unstable jujube nectar output and short supply of jujube nectar in the market.

Inactive Publication Date: 2016-01-06
NORTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the production of honey is easily affected by the geographical location of the plant, the flowering period of the plant, the climate, the strength o

Method used

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  • Characteristic protein for identifying Chinese date honey flower source
  • Characteristic protein for identifying Chinese date honey flower source
  • Characteristic protein for identifying Chinese date honey flower source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 honey crude protein

[0030] Weigh 5g of honey samples respectively, dissolve them in 5ml of distilled water, mix well, centrifuge at 5000g for 10 minutes, collect the supernatant, dilute to 10ml, and store in a refrigerator at 4°C for later use.

Embodiment 2

[0031] The separation of embodiment 2 jujube nectar characteristic protein

[0032] (1) Loading Take 2 suitable clean and dry glass plates, assemble them into pouring flat film, clamp them with strong clamps and put them on the flat film seat;

[0033] (2) 12% separating gel and 5% stacking gel were used for gel loading. The specific formula of the gel buffer system is shown in Table 1. Prepare the separating gel solution according to Table 1, mix gently, and immediately pour the gel solution into the assembled slab gel membrane through a constant flow pump until the height of the separating gel solution is 3.5 cm from the top of the glass plate. Then add a layer of water on the surface of the separation gel to seal the surface of the gel to promote polymerization and make the surface of the separation gel straight. After gelling at room temperature for 40 minutes to an hour, a layer of interface can be seen, indicating that the gel has been completely gelled. Aspirate off...

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Abstract

The invention relates to a Chinese date honey characteristic protein and isolation and purification thereof; firstly, eight single-flower honey is subjected to SDS-PAGE, a result finds out that the Chinese date honey has one protein spectral band at about 17 ku while protein electrophoresis spectrograms of other honeys do not contain the protein spectral band at about 17 ku; in addition, the molecular weight of the protein has quite large difference from the molecular weights of other protein spectral bands, so as to facilitate separation. Therefore, the protein can be used as the characteristic protein of the Chinese date honey different from other honeys. For making the molecular weight and structure information of the protein clear, an LC-Chip/ESI-QTOF-MS method is used for identification. The operation is simple, the separation effect is good, the feasibility is high, and the new method is provided for true and false identification of the Chinese date honey.

Description

technical field [0001] The invention relates to a characteristic protein for identifying jujube nectar and its separation and purification, belonging to the field of food science. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, mix with their own secretions, and fully brew. Honey is rich in nutrients and complex in composition, containing a variety of essential amino acids, vitamins, phenolic compounds, flavonoids, etc., so it has a variety of biological activities, such as anti-oxidation, antibacterial, accelerating wound repair and improving immunity. Anemia and high blood pressure have a certain auxiliary curative effect. Because the sensory characteristics of honey are easily affected by factors such as processing, storage and crystallization, the content of the main components of different types of honey is not significant, and it is difficult to distinguish honey varieties by traditio...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/26G01N33/68
CPCC07K14/43572G01N33/68G01N2333/43565
Inventor 曹炜陈妮周厚报高慧
Owner NORTHWEST UNIV
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