Characteristic protein for identifying Chinese date honey flower source

Abstract

The invention relates to a Chinese date honey characteristic protein and isolation and purification thereof; firstly, eight single-flower honey is subjected to SDS-PAGE, a result finds out that the Chinese date honey has one protein spectral band at about 17 ku while protein electrophoresis spectrograms of other honeys do not contain the protein spectral band at about 17 ku; in addition, the molecular weight of the protein has quite large difference from the molecular weights of other protein spectral bands, so as to facilitate separation. Therefore, the protein can be used as the characteristic protein of the Chinese date honey different from other honeys. For making the molecular weight and structure information of the protein clear, an LC-Chip / ESI-QTOF-MS method is used for identification. The operation is simple, the separation effect is good, the feasibility is high, and the new method is provided for true and false identification of the Chinese date honey.

Description

technical field [0001] The invention relates to a characteristic protein for identifying jujube nectar and its separation and purification, belonging to the field of food science. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, mix with their own secretions, and fully brew. Honey is rich in nutrients and complex in composition, containing a variety of essential amino acids, vitamins, phenolic compounds, flavonoids, etc., so it has a variety of biological activities, such as anti-oxidation, antibacterial, accelerating wound repair and improving immunity. Anemia and high blood pressure have a certain auxiliary curative effect. Because the sensory characteristics of honey are easily affected by factors such as processing, storage and crystallization, the content of the main components of different types of honey is not significant, and it is difficult to distinguish honey varieties by traditio...

AI Technical Summary

Problems solved by technology

However, the production of honey is easily affected by the geographical location of the plant, the flowering period of the plant, the climate, the strength of the bee colony, and the time for collecting honey, which makes the output of jujube honey unstable, so that the market for jujube honey is in short supply.

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