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Method for extracting urokinase and ulinastatin from urine

A technology of ulinastatin and urokinase, which is applied in the field of extracting urokinase and ulinastatin, can solve the problems of low activity, waste, and affecting the economic value of urine, and achieve the effect of improving economic value, increasing production and quality

Inactive Publication Date: 2016-01-13
张昭
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Problems solved by technology

[0002] In recent years, there have been many companies and individuals extracting crude proteases such as urokinase and ulinastatin from male urine, but there are generally poor techniques for extracting crude products of urokinase and ulinastatin, resulting in the loss of urokinase and ulinastatin The extraction amount is small and the activity is low, causing a lot of waste and affecting the economic value of urine

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  • Method for extracting urokinase and ulinastatin from urine

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Embodiment Construction

[0032] see figure 1 As shown, the present invention provides a method for extracting urokinase and ulinastatin from urine, comprising the following steps from S101 to S106, specifically:

[0033] S101: Collect urine and adjust its pH value to 6.0-6.5

[0034] In a preferred embodiment of the present invention, the "S101: Collect urine and adjust its pH value to 6.0-6.5" specifically includes: collecting urine using a non-flush urinal and pipes; adjusting the pH of the urine with hydrochloric acid; pH value to 6.3. In this example, the urine is injected into the first-generation stirring tank, and dilute hydrochloric acid (the ratio of hydrochloric acid to water is 1:5) is slowly injected while stirring to adjust the pH value of the urine until the pH value is stable at 6.3.

[0035] S102: Add powdered silica gel to the urine and stir, so that the urokinase protein in the urine is adsorbed by the silica gel

[0036] In a preferred embodiment of the present invention, the...

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Abstract

The invention provides a method for extracting urokinase and ulinastatin from urine. The method comprises the following steps: collecting the urine, and adjusting the pH value of the urine; adding silica gel powder into the urine, and stirring so that urokinase protein in the urine is adsorbed by the silica gel; acquiring the silica gel through a filtering method, and adjusting the pH value of the filtered urine; adding chitin into the filtered urine so that ulinastatin protein in the urine is adsorbed by the chitin; acquiring urokinase protein fluid and ulinastatin protein fluid from the silica gel and the chitin, respectively; and adding the urokinase protein fluid into saturated water, and extracting to obtain urokinase, and adding ammonium sulfate into the ulinastatin protein fluid, and extracting to obtain ulinastatin. According to the method provided by the invention, the urokinase crude product and the ulinastatin crude product can be extracted from the urine, so that the economic value of the urine is greatly improved.

Description

technical field [0001] The invention relates to biological extraction technology, in particular to a method for extracting urokinase and ulinastatin from urine. Background technique [0002] In recent years, there have been many companies and individuals extracting crude proteases such as urokinase and ulinastatin from male urine, but there are generally poor techniques for extracting crude products of urokinase and ulinastatin, resulting in the loss of urokinase and ulinastatin The extraction amount is small and the activity is low, causing a lot of waste and affecting the economic value of urine. Therefore, how to improve the extraction yield and quality of crude urokinase and ulinastatin is a very promising research. This research can turn the collected urine into treasure and further increase the economic value of urine. Contents of the invention [0003] The technical problem solved by the present invention is to provide a method for extracting urokinase and ulinast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/72C07K14/81
CPCC12N9/6462C07K14/81C12Y304/21073
Inventor 张昭
Owner 张昭
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