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Method for extracting ferritin from spinach

A ferritin and spinach technology, which is applied in protein food processing, vegetable protein processing, protein food ingredients, etc., can solve the problem that iron supplementation has little effect and cannot achieve iron supplementation.

Inactive Publication Date: 2016-01-20
徐鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the amount of iron in spinach is very small, eating spinach can not achieve the purpose of iron supplementation. If eating spinach can achieve the purpose of iron supplementation, then a person needs to eat 100 catties or more at a time to achieve the purpose of iron supplementation. amount, therefore, the effect of iron supplementation by eating spinach is negligible

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In an embodiment of the present invention, a method for extracting ferritin from spinach comprises the following steps:

[0022] (1) First remove the yellow leaves and insect bites from the collected spinach, rinse it with clean water, soak it in an alcohol solution containing 70%-75%, then drain the alcohol solution on a sieve, and put it in a sterile Wash away the alcohol solution in water, drain it on the sieve, and then wash it again with sterile water to ensure that there is no alcohol solution residue;

[0023] (2) In order to reduce microbial contamination, the sterilized and cleaned spinach is immediately sent to the sterile working room for follow-up treatment. First, cut the spinach into 1-2cm pieces with a grass cutter, and then put it into a juicer for squeezing Juice, after squeezing the juice, add cellulase and pectinase, mix well, place at 45°C for enzymatic reaction, keep the temperature constant during the reaction, and last for 35 minutes;

[0024] (3...

Embodiment 2

[0031] In an embodiment of the present invention, a method for extracting ferritin from spinach comprises the following steps:

[0032] (1) First remove the yellow leaves and insect bites from the collected spinach, rinse it with clean water, soak it in an alcohol solution containing 70%-75%, then drain the alcohol solution on a sieve, and put it in a sterile Wash away the alcohol solution in water, drain it on the sieve, and then wash it again with sterile water to ensure that there is no alcohol solution residue;

[0033] (2) In order to reduce microbial contamination, the sterilized and cleaned spinach is immediately sent to the sterile working room for follow-up treatment. First, cut the spinach into 1-2cm pieces with a grass cutter, and then put it into a juicer for squeezing Juice, after squeezing the juice, add cellulase and pectinase, mix evenly, place at 55°C for enzymatic reaction, keep the temperature constant during the reaction, and last for 25 minutes;

[0034] ...

Embodiment 3

[0041] In an embodiment of the present invention, a method for extracting ferritin from spinach comprises the following steps:

[0042] (1) First remove the yellow leaves and insect bites from the collected spinach, rinse it with clean water, soak it in an alcohol solution containing 70%-75%, then drain the alcohol solution on a sieve, and put it in a sterile Wash away the alcohol solution in water, drain it on the sieve, and then wash it again with sterile water to ensure that there is no alcohol solution residue;

[0043] (2) In order to reduce microbial contamination, the sterilized and cleaned spinach is immediately sent to the sterile working room for follow-up treatment. First, cut the spinach into 1-2cm pieces with a grass cutter, and then put it into a juicer for squeezing Juice, after squeezing the juice, add cellulase and pectinase, mix evenly, place at 50°C for enzymatic reaction, keep the temperature constant during the reaction, and last for 30 minutes;

[0044] ...

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PUM

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Abstract

The invention discloses a method for extracting ferritin from spinach. The method includes performing surface sterilization of collected fresh spinach, juicing the spinach, performing enzymolysis, precipitating protein, performing centrifugal separation, dissolving, and dialyzing to get the final products. The iron content of the ferritin finished products prepared by the invention can reach 31.6 mg / kg. The iron of the spinach can be fully extracted, and the problem of difficulty of iron supplementation for anemia patients is overcome.

Description

technical field [0001] The invention relates to biological extraction technology, in particular to a method for extracting ferritin from spinach. Background technique [0002] Iron is an essential element for almost all living organisms. The content of iron ranks fourth in the earth's crust, but its content in organisms is very low, which is related to the fact that ferric ions are easy to form insoluble compounds, which leads to the reduction of the absorption and utilization of iron by organisms. Ferritin is an iron-binding and storage protein that widely exists in animals, plants and microorganisms. It is a highly conserved multifunctional subunit protein in organisms. [0003] Spinach is also called Persian vegetable and red root vegetable. It belongs to cold-resistant green leafy vegetables and is one of the main vegetables supplied in early spring and off-season in my country. Spinach is known as "Nutrition Model Vegetable". It is rich in carotenoids, vitamin C, iron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J3/14A23J3/34
Inventor 徐鹏
Owner 徐鹏