Dicranostigma leptopodum (maxim) fedde alkaloid effective part preparation method
A technology of effective parts and flower organisms, applied in the field of natural product chemistry, can solve the problems of high dosage of extracts, large daily dosage of patients, and insignificant biological activity, so as to reduce the toxicity in vivo, reduce the content and separate impurities. full effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] Example 1: Extraction and separation of effective parts of alkaloids in Tuchuang flower
[0038] A, acid water extraction step: 4Kg of bald sore flower medical material is pulverized, add 60L concentration and be the sulfuric acid aqueous solution of 0.05%, place 4h, reflux extraction 1h, filter, add 40L concentration of medicine dregs and be the sulfuric acid aqueous solution of 0.05%, reflux extraction 2 times, Extract for 1 hour each time, filter, combine the three filtrates, use 10% NaOH solution to neutralize the value to neutral, recover the solvent under reduced pressure, concentrate to obtain 6.5 L of alkaloid extract of bald acne flower, and detect by HPLC-DAD.
[0039] B. Alcohol precipitation and impurity removal step: in step A, add 20L of 95% ethanol to the alkaloid extract of bald sore flower under mechanical stirring, stir for 1h, leave it for 4h naturally, filter and remove impurities through Buchner funnel, and recover the solvent under reduced pressure ...
Embodiment 2
[0044] Embodiment 2: Extraction and separation of effective parts of alkaloids in Tuchuang flower
[0045] A, acid water extraction steps: 4Kg of the bald sore flower medicinal material is crushed, add 40L of 0.01% aqueous hydrochloric acid solution, stand for 24h, reflux for 1h, extract 3 times, filter, discard the medicinal residues, combine the filtrates, and neutralize with NaOH solution value to neutral, the solvent was recovered under reduced pressure, concentrated to 4L, and sampled for HPLC-DAD detection.
[0046] B. Alcohol precipitation and impurity removal step: in step A, add 20L of methanol to the alkaloid extract of bald sore flower under mechanical stirring, stir for 1 hour, let it stand naturally for 4 hours, centrifuge and filter to remove impurities, recover the solvent from the mother liquor under reduced pressure until viscous, add water Dissolved to obtain 5L of the purified product of bald acne flower alkaloids.
[0047] C. Extraction and separation step...
Embodiment 3
[0051] Example 3: Extraction and separation of effective parts of alkaloids in Tuchuang flower
[0052] A, acid water extraction steps: 4Kg of bald sore flower medicinal material is crushed, add 40L concentration of 0.01% sulfuric acid aqueous solution, place to soak for 4h, reflux extraction for 1h, filter, and reflux extract 2 times with 40L concentration of 0.01% sulfuric acid aqueous solution for medicinal residues , 1 hour each time, combine three filtrates, use NaOH solution to neutralize the value to neutral, recover the solvent under reduced pressure, concentrate to 4L, and sample for HPLC-DAD detection.
[0053] B. Alcohol precipitation and impurity removal step: in step A, add 20L of 95% ethanol to the alkaloid extract of bald sore flower under mechanical stirring, stir for 1 hour, let it stand naturally for 4 hours, centrifugally filter and remove impurities, and recover the solvent from the mother liquor under reduced pressure to no alcohol taste, to obtain 5L of p...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com