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Graded extraction method for subcomponents of fulvic acid in soil

A fulvic acid sub-fractional extraction technology, which is applied in the field of fractional extraction of soil fulvic acid sub-components, can solve the problem that the extraction process and the grading process of fulvic acid sub-components cannot be unified, and the occurrence state and physical and chemical properties of fulvic acid cannot be reflected. nature, etc.

Inactive Publication Date: 2016-01-20
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

The previous classification process of fulvic acid subcomponents has nothing to do with the state of fulvic acid in the soil, and cannot reflect the occurrence state and physical and chemical properties of fulvic acid in soil; nor can it realize the unity of the extraction process and classification process of fulvic acid subcomponents

Method used

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Examples

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Embodiment 1

[0086] Embodiment 1: Utilize the method of the present invention to extract fulvic acid subcomponents in the surface soil of Beijing Mangshan Forest, and prepare different levels of fulvic acid subcomponents graded samples, the specific operations are as follows:

[0087] (1) Test reagents and samples:

[0088] Soil sample: surface soil of Mangshan forest in Beijing;

[0089] Hydrochloric acid: analytically pure; sodium hydroxide: analytically pure; sodium pyrophosphate: analytically pure; XAD-8 resin: Sigma.

[0090] (2) Extraction steps:

[0091] Step a: Soil sample pretreatment: Weigh 10Kg of natural soil, remove tree roots and stones and other debris, air-dry, and grind through a 2.0mm sieve to obtain a pretreated soil sample;

[0092] Step b: Fractional extraction of soil organic matter:

[0093] Add 100L of deionized water to the pretreated soil sample in step a to make the solid-to-liquid ratio reach 1:10, stir continuously for 4h, and after standing for 24h, centrif...

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Abstract

The invention discloses a graded extraction method for subcomponents of fulvic acid in soil. The extraction method comprises the following steps: step a, pretreatment of a soil sample; step b, graded extraction of organic matters in soil; step c, preparation of crude graded subcomponents of fulvic acid; step d, concentration of the crude graded subcomponents of fulvic acid; and step e, purification of subcomponent samples of fulvic acid. Thus, the method provided by the invention can extract fulvic acid subcomponent solutions by using different solution continuous extraction technologies and enrich the fulvic acid subcomponent solutions extracted in grades by using macroporous resin, and thus, graded fulvic acid subcomponent solid samples are obtained.

Description

technical field [0001] The invention relates to the technical field of soil separation and extraction, in particular to a method for fractional extraction of soil fulvic acid subcomponents. Background technique [0002] Fulvic acid is a macromolecular organic mixture formed by the degradation of biomass, which is widely distributed in environmental media such as water, soil, and air. The structure of fulvic acid is very complex, the molecular weight ranges from hundreds to hundreds of thousands, and the elemental composition and chemical structure vary with time, space and source. Fulvic acid plays an important role in environmental geochemistry and global ecosystems. Countries around the world have carried out extensive research on the geochemical characteristics of fulvic acid from various sources such as oceans, rivers, and wastewater. At present, some progress has been made in the overall structural characteristics and geochemical cycle of fulvic acid, but due to the ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/40
Inventor 白英臣吴丰昌赵晓丽宋凡浩陈曲
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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