Preparation method of a traditional Chinese medicine composition for preventing and treating bacterial enteritis in aquatic animals

Abstract

The invention discloses a preparation method of a traditional Chinese medicinal composition for preventing and treating bacterial enteritis in aquatic animals. The preparation method specifically comprises the following steps: (1) implementing superfine grinding on herba portulacae, Chinese pulsatilla root and cortex phellodendri, conducting heating and water extraction; filtering and reserving a solution, and concentrating; (2) heating and preparing a chitosan and glacial acetic acid solution; (3) mixing a Chinese medicinal extract with the chitosan and glacial acetic acid solution; (4) emulsifying a mixture in an emulsifying system; (5) cross-linking an emulsified product; and (6) cross-linking a reaction product through washing, precipitating, centrifuging and drying so as to obtain a chitosan microsphere loaded with a traditional Chinese medicine extract. The preparation method is simple, and the preparation method is adaptive to demands of large-scale industrial production.

Description

technical field

[0001] The invention relates to a preparation method of a traditional Chinese medicine composition, in particular to a preparation method of a traditional Chinese medicine composition for preventing and treating bacterial enteritis of aquatic animals. Background technique

[0002] Fish bacterial enteritis is the most common fish disease. The pathogen is Aerobacillus punctatus, which is an opportunistic pathogen and mainly harms grass carp, herring, silver carp, bighead carp and other aquatic animals. Grass carp and herring may be infected from fingerlings to adult fish. The mortality rate of the disease is high, generally about 50%, and the mortality rate in fish ponds with severe disease can be as high as 90%. Generally, bacterial enteritis of fish begins to be popular when the water temperature is above 18°C, and the epidemic peak is when the water temperature is 25-30°C. There are two obvious epidemic seasons in a year. From May to June, grass carp and her...