Sample processing apparatus of atomic force microscope and biological sample preparation method

Abstract

The invention discloses a sample processing apparatus of atomic force microscope and a biological sample preparation method. The sample processing apparatus comprises an immersion pool and a sample pool; and the sample pool is arranged right above the immersion pool. A plurality of concave first unit pools are arranged on the sample pool uniformly; the lower end of each first unit pool protrudes from the sample pool; a serial number is arranged on each first unit pool; and a bottom hole is formed in the bottom of each first unit pool and an air hole is formed in the side of each first unit pool. A first object placing hole is formed in one side of the sample pool; and a temperature sensor is arranged in the first object placing hole in an embedded mode. A second object placing hole is formed in the other end, opposite to the first object placing hole, of the sample pool; and a heating rod is arranged in the second object placing hole in an embedded mode. According to the technical scheme, the structure is designed reasonably and simply; a plurality of samples can be prepared automatically by one-time operation; the processed flow is consistent with all experiment parameters; and the preparation efficiency and experiment repeatability are improved.

Description

technical field [0001] The invention relates to the technical field of sample processing devices, in particular to an atomic force microscope sample processing device and a biological sample preparation method. Background technique [0002] Atomic force microscopy has been widely used to image various samples, especially biological samples represented by DNA. The general biological sample preparation method usually uses a flat surface such as mica or silicon wafer as a base to deposit samples on it, and sticks it on a metal iron sheet with a diameter of about 1 cm and puts it on the sample stage of an atomic force microscope for scanning. The preparation of biological samples includes processes such as deposition, cleaning, and nitrogen blow-drying. There are many steps, and the details of each process such as deposition time, cleaning time, and nitrogen flow rate must be well controlled. Most of the existing technical solutions use manual operations, and generally one expe...

AI Technical Summary

Problems solved by technology

Existing technical solutions mostly use manual operation. Generally, multiple samples need to be prepared for one experiment, and each sample can only be prepared sequentially.

The preparation efficiency of this conventional method is relatively low, and it is difficult to control the process parameters of each operation to be strictly consistent, resulting in poor repeatability. Even if the samples used are the same, the scan results obtained are often different, resulting in analysis and comparison. difficulty

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