An atomic force microscope sample processing device and biological sample preparation method

Abstract

The invention discloses an atomic force microscope sample processing device and a biological sample preparation method, comprising a dipping pool and a sample pool, the sample pool is arranged directly above the dipping pool, and several concave first units are uniformly arranged on the sample pool pool, the lower end of each first unit pool protrudes from the bottom surface of the sample pool, each first unit pool is provided with a number, and the bottom surface of each first unit pool is provided with a bottom hole, the bottom of each first unit pool There is an air hole on the side, a first storage hole is opened on one side of the sample pool, a temperature sensor is embedded in the first storage hole, and a second storage hole is provided at the other end of the sample pool relative to the first storage hole , a heating rod is embedded in the second storage hole. The above-mentioned technical solution has a reasonable structural design and a simple structure, and can automatically prepare multiple samples at one time, and ensure that the processing flow is consistent with various experimental parameters, thereby improving its preparation efficiency and experimental repeatability.

Description

technical field [0001] The invention relates to the technical field of sample processing devices, in particular to an atomic force microscope sample processing device and a biological sample preparation method. Background technique [0002] Atomic force microscopy has been widely used to image various samples, especially biological samples represented by DNA. The general biological sample preparation method usually uses a flat surface such as mica or silicon wafer as a base to deposit samples on it, and sticks it on a metal iron sheet with a diameter of about 1 cm and puts it on the sample stage of an atomic force microscope for scanning. The preparation of biological samples includes processes such as deposition, cleaning, and nitrogen blow-drying. There are many steps, and the details of each process such as deposition time, cleaning time, and nitrogen flow rate must be well controlled. Most of the existing technical solutions use manual operations, and generally one expe...

AI Technical Summary

Problems solved by technology

Existing technical solutions mostly use manual operation. Generally, multiple samples need to be prepared for one experiment, and each sample can only be prepared sequentially.

The preparation efficiency of this conventional method is relatively low, and it is difficult to control the process parameters of each operation to be strictly consistent, resulting in poor repeatability. Even if the samples used are the same, the scan results obtained are often different, resulting in analysis and comparison. difficulty

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