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Bladder cancer screening detection kit

A detection kit and bladder cancer technology, which is applied in the field of bladder cancer detection and treatment, can solve the problems of bladder cancer, such as unclear connection, and achieve the effect of promoting apoptosis and inhibiting proliferation

Inactive Publication Date: 2016-02-17
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

PVT1 is a star member of the long-chain non-coding family and plays an important role in prostate cancer, ovarian cancer, gastric cancer, liver cancer and other cancers, but the relationship between this gene and bladder cancer is still unclear

Method used

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Embodiment Construction

[0019] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0020] Technology and Method

[0021] 1. Cell culture

[0022] Bladder cancer cells T24, 5637 were purchased from American Type Culture Collection (ATCC, Manassas, USA). Normal bladder epithelial immortalized cells SV-HUC-1 were purchased from Shanghai Cell Research Institute. T24 was cultured in a cell culture medium consisting of 90% DMEM (Invitrogen, CA), 10% fetal bovine serum (Invitrogen), 1%-2% glutamine and 0.5%-1% double antibody. 5637 was cultured in a cell culture medium consisting of 90% 1640 (Invitrogen, CA), 10% fetal bovine serum (Invitrogen), 1%-2% glutamine and 0.5%-1% double antibody. SV-HUC-1 in cells composed of 90% F-12K (Invitrogen, CA), 10% fetal bovine serum (Invitrogen), 1%-2% glutamine and 0.5%-1% double antibody Cultivated in culture medium. All three cell lines were placed at 37°C, 5% CO 2 incubato...

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Abstract

The invention discloses a bladder cancer screening detection kit which is capable of recognizing PVT1 genes specifically. The bladder cancer screening detection kit has the advantages that PVT1 is highly expressed in bladder cancer cells, and by means of detecting the PVT1 genes, early screening of the bladder cancer can be achieved; tetracycline-induced PVT1 shRNA (short hairpin ribonucleic acid) is capable of knocking out PVT1 expression effectively, inhibiting bladder cancer cell multiplication and promoting bladder cancer cell apoptosis so as to make bladder cancer diagnosis and treatment possible.

Description

technical field [0001] The present application relates to the field of bladder cancer detection and treatment. Background technique [0002] Some scientific studies have shown that long non-coding RNAs (lncRNAs) larger than 200 nucleotides are involved in the progression of various human diseases, especially tumors. PVT1 is a star member of the long-chain non-coding family and plays an important role in prostate cancer, ovarian cancer, gastric cancer, liver cancer and other cancers, but the relationship between this gene and bladder cancer is still unclear. The tetracycline-inducible system is the most widely used regulatory system today and is a tool that has played a huge role in the emerging era of medical synthetic biology. This system can be used to control gene expression, providing us with new ideas for treating bladder cancer. Contents of the invention [0003] The invention provides a novel bladder cancer screening and detection kit. [0004] The invention prov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K48/00A61K31/713A61P35/00C12N15/113
CPCA61K31/713C12N15/113C12N2310/14C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 蔡志明庄成乐刘宇辰黄卫人
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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