Bladder cancer screening detection kit
A detection kit and bladder cancer technology, which is applied in the field of bladder cancer detection and treatment, can solve the problems of bladder cancer, such as unclear connection, and achieve the effect of promoting apoptosis and inhibiting proliferation
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[0019] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.
[0020] Technology and Method
[0021] 1. Cell culture
[0022] Bladder cancer cells T24, 5637 were purchased from American Type Culture Collection (ATCC, Manassas, USA). Normal bladder epithelial immortalized cells SV-HUC-1 were purchased from Shanghai Cell Research Institute. T24 was cultured in a cell culture medium consisting of 90% DMEM (Invitrogen, CA), 10% fetal bovine serum (Invitrogen), 1%-2% glutamine and 0.5%-1% double antibody. 5637 was cultured in a cell culture medium consisting of 90% 1640 (Invitrogen, CA), 10% fetal bovine serum (Invitrogen), 1%-2% glutamine and 0.5%-1% double antibody. SV-HUC-1 in cells composed of 90% F-12K (Invitrogen, CA), 10% fetal bovine serum (Invitrogen), 1%-2% glutamine and 0.5%-1% double antibody Cultivated in culture medium. All three cell lines were placed at 37°C, 5% CO 2 incubato...
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