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Compound capable of serving as Wnt signal pathway activator and preparation and application thereof

A compound and signal technology, applied in the direction of medical preparations containing active ingredients, anti-inflammatory agents, drug combinations, etc., can solve problems such as no obvious progress in research

Active Publication Date: 2016-02-24
CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the Wnt / β-catenin signaling pathway is related to many cancers and diseases, the out-of-control Wnt / β-catenin signaling pathway can be used as a good way to treat diseases related to the Wnt / β-catenin signaling pathway, but at this stage There has been no significant progress in the research

Method used

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  • Compound capable of serving as Wnt signal pathway activator and preparation and application thereof
  • Compound capable of serving as Wnt signal pathway activator and preparation and application thereof
  • Compound capable of serving as Wnt signal pathway activator and preparation and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] 1. Experimental materials

[0081] 1) Plasmid

[0082] TOPFlash plasmid was purchased from Millipore Corporation.

[0083] GFP plasmid was purchased from Millipore Corporation.

[0084] 2) Antibody

[0085] β-actin (sc-47778) antibody was purchased from Santa Cruz; SP1 (S9809) antibody was purchased from Sigma; β-catenin (610154) antibody was purchased from BD Biosci.Pharmingen.

[0086] 3) Reagents

[0087] Protease inhibitor (complete protease inhibitor mixed tablet) and NP-40 were purchased from Roche Company; Lipofectamine and PLUS transfection reagent were purchased from Invitrogen Company;

[0088] Wnt3a conditioned medium was prepared from Wnt3a cell line (CRL-2647, purchased from ATCC Cell Bank, USA): Wnt3a cells were cultured in DMEM cell medium supplemented with 10% calf serum for 4 days, and then the supernatant medium was collected by centrifugation.

[0089] Control conditioned medium was prepared from L cell line (CRL-2648, purchased from ATCC Cell Bank...

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Abstract

The invention provides a compound serving as a Wnt signal pathway activator and preparation and application thereof. A compound with the structural formula shown in a formula (I) or a formula (II) is adopted as the compound. The compound can be used for preparing medicine for adjusting a classic Wnt signal pathway and researching the classic Wnt signal pathway. In addition, new thought is supplied to screening of the medicine for adjusting the Wnt signal pathway and medicine for preventing and treating related diseases of the classic Wnt signal pathway and researching of application of the Wnt signal pathway in stem cells.

Description

technical field [0001] The present invention relates to a compound that can be used as an activator of Wnt signaling pathway and its preparation and application. Background technique [0002] The Wnt signal transduction pathway is a highly conserved signal transduction pathway in the evolution of organisms, regulating and controlling many life activities. In the early development of animals, Wnt signaling determines a series of important events such as the formation of the dorsal-ventral axis, germ layer establishment, somite differentiation, tissue or organ formation, and directly controls proliferation, differentiation, polarization, apoptosis and anti-apoptosis The fate of cells [Clevers, H. and R. Nusse, Wnt / beta-Catenin Signaling and Disease. Cell, 2012.149(6): p.1192-205]. More than a dozen members of Wnt proteins are involved in different signal transduction pathways by interacting with different receptors on the cell membrane. These pathways are mainly divided into...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4741A61P19/02A61P29/00A61P19/10A61P7/00
CPCA61K31/4741C07D491/056C07D519/00
Inventor 李林汪胜郝小江潘巍峻尹俊林聂芬陈铎之
Owner CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI