Molecular marking method for major gene locus qRL1 for length of adventitious roots in cuttage of catalpa bungei

A main effect gene and molecular marker technology, applied in the field of molecular genetics, to achieve the effects of reducing breeding costs, accelerating promotion, and shortening the breeding cycle

Inactive Publication Date: 2016-03-02
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Molecular marking method for major gene locus qRL1 for length of adventitious roots in cuttage of catalpa bungei

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[0008] Statistical analysis of adventitious root length of Catalpa cuttings: In early March 2013 and early March 2014, 87 five-year-old branches of Catalpa clones were cut into 30 cm long and placed evenly in a flat sand bed, and then covered with 3 ~5cm of fine sand, pour water thoroughly. Spray and irrigate the sand bed with 500 times of carbendazim turbid solution every week. Mix peat and perlite at a volume ratio of 1:1, put them into seedling pots (15×15×20cm), and spray and water them with 500 times carbendazim. When the height of the newly germinated shoots in the sand bed reaches 10-15 cm, remove the tops of the shoots, and after a week, remove the shoots with the heels as cuttings. Cut off the leaves on the cuttings together with the petiole, leaving only the top leaf, and cut it in half. Dip the base of the treated cuttings in 75% alcohol to disinfect for 30 seconds, rinse them with clean water, then dip them in 3,000 mg / L IBA for 1 minute, insert the cuttings into...

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Abstract

The invention provides a molecular marking method for the major gene locus qRL1 for the length of adventitious roots in cuttage of catalpa bungei and belongs to the field of molecular genetics. 70 pairs of SSR molecule markers are used for determining 87 parts of clonal gene types of catalpa bungei, and whole genome association linkage analysis is carried out with the combination of the length of the adventitious roots in cuttage of catalpa bungei. The major gene locus qRL1 for the length of the adventitious roots in cuttage of catalpa bungei is detected, 12.54% of phenotypic variation is explained, and the SSR molecular marker CB128 is highly remarkably associated with phenotypic variation. The molecular marking method is beneficial to solving the problem of the slow progress of catalpa bungei breeding in China and beneficial to solving the technical problem that the existing breeding technology is high in breeding cost, long in time, poor in stability and the like, and breeding of new varieties of catalpa bungei in China and clone forestry development can be accelerated.

Description

technical field [0001] The invention provides a molecular marker method for the main effect gene site qRL1 of adventitious root length of catalpa cuttings, belongs to the field of molecular genetics, and is specially used for directional cultivation of new catalpa species with high rooting ability of catalpa cuttings. Background technique [0002] Catalpa ( Catalpa bungei ) belongs to Bignoniaceae ( Bignoniaceae ) Catalpa ( Catalpa ) is an excellent and precious timber tree species. With the development of industry and the growth of the world population, the demand for wood is increasing day by day, and the shortage of wood has become a worldwide problem, especially in my country. The development of clonal forestry has become an effective way to alleviate the shortage of timber in my country, but the rooting rate of cuttings is the bottleneck restricting the development of clonal forestry. Catalpa is a tree species with low cutting rooting rate and few roots. Difficult...

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 王鹏杨如同李林芳李亚马玲玲王淑安汪庆
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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