Pharmaceutical composition
A composition and drug technology, applied in the field of mixed extracts of Atractylodes Rhizoma Atractylodes Rhizome and Dandelion, can solve the problem of no reliable treatment method for inflammatory bowel disease, and achieve the goal of preventing and treating inflammatory bowel disease, preventing or treating inflammatory bowel disease Effect
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Embodiment 1
[0058] Embodiment 1: Preparation of the mixed extract of Atractylodes macrocephala and Dandelion
[0059] Preparation of mixed extract of Atractylodes macrocephala and Dandelion
[0060] 10 times the weight of water was added to 50 g of Atractylodes macrocephala (ie, derived from Atractylodes macrocephala, country of origin: China, purchased from Daewoo Herbal Medicine Wholesaler), and the extraction was repeated twice at 100° C. within 3 hours. By concentrating the above extracted filtrate under reduced pressure (ie, 40 brix) and lyophilizing it, 19.4 g of a powdery Atractylodes Rhizome extract was prepared (yield: 38.8%).
[0061] Dandelion (country of origin: China, purchased from Daewoo herbal medicine wholesaler) extract was prepared in the same way as Atractylodes macrocephala. Use 50g dandelion to prepare the dandelion extract that obtains 8.48g (yield: 17.0%)
[0062] A mixed extract was prepared by mixing the prepared Atractylodes macrocephala extract and Dande...
Embodiment 2
[0069] Example 2: Confirmation of the therapeutic effect of the mixed extract of Atractylodes macrocephala and Dandelion on inflammatory bowel disease
[0070] Ulcerative colitis was induced by allowing 8-week-old male mice (C57BL / 6, Samtako BIO Korea Inc.) to drink 5% aqueous dextran sulfate sodium (DSS) solution voluntarily for 7 days.
[0071] According to the administration of DSS, the therapeutic effect on inflammatory bowel disease of administering the mixed extract of Atractylodes macrocephala and Dandelion after the induction of ulcerative colitis was confirmed. The experimental groups and the extract administered to each experimental group are given in Table 2 below.
[0072] [Table 2]
[0073]
[0074] The drug was administered orally twice a day for 7 days, and the environment was controlled at a temperature of 22 ± 2°C, a relative humidity of 50 ± 10% and a cycle of lighting (illumination cycle, that is, lights on at 07:00 / lights off at 19:00) . During the ...
Embodiment 3
[0079] Example 3: Confirmation of histological changes after administration of mixed extracts of Atractylodes macrocephala and Dandelion
[0080] Changes in intestinal tissue were confirmed by using the intestine obtained in Example 2.
[0081] Experimental animals were sacrificed; colon tissue was removed; it was rinsed with PBS and fixed with buffered formalin. Then, they were embedded in paraffin by ethanol dehydration, and sectioned. Stained with hematoxylin-eosin (H&E) dye and visualized by microscopy.
[0082] The results are shown in image 3 middle. Such as image 3 As shown, intestinal tissue is unclear due to disrupted cryptalarea and goblet cells in different regions of the mucosal epithelial layer. However, compared with the control group, the intestinal tissue structure of the mixed extract of Atractylodes macrocephala and Dandelion (30 mg / kg, 100 mg / kg) administration group of Example 1 is clear, and it has been confirmed that this group exhibits similarit...
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