A kind of Penicillium grisea and its application for preventing and treating fungal diseases
A technology of Penicillium grisea and Sclerotinia, which is applied in the direction of application, fungi, fungicides, etc., to achieve the effects of simple production and use, low cost, and inhibition of occurrence and spread
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Embodiment 1
[0042] Isolation and Identification of P417 Strain
[0043] 1. Isolation location, isolation method (morphological identification of traditional microbiology)
[0044] Microbial characteristics
[0045] Isolation of P417 strain: The applicant isolated and screened a Penicillium strain with strong control effect on Botrytis cinerea from rape plants collected in Qinghai Province, China, and the applicant named it P417. The separation and screening of P417 bacterial strain of the present invention adopt methods such as dilution plate method, plate antagonism, in vitro test, strain identification work with reference to " fungus identification handbook " (Wei Jingchao, 1979), " morphology and classification of fungi " (Dai Fanglan, 1987) ) and other monographs of the test methods. The P417 strain was cultured at 25°C for 5 days, the genomic DNA was extracted by the CTAB method (Taylor et al 1993), and the rDNA-ITS region was amplified by using fungal ribosomal DNA universal prime...
Embodiment 2
[0056] P417 strain and Botrytis cinerea plate confrontation test:
[0057] Use a puncher with an inner diameter of 9mm to punch the activated Botrytis cinerea and connect it to one side of the PDA plate, and the other side to connect the P417 strain (9mm inner diameter). Botrytis cinerea was inoculated and the other side was not inoculated as a control, and repeated 3 times. After 3-4 days, observe the magnitude of its antagonistic effect and measure the width of its bacteriostatic zone. See the test results Figure 4 ,Table 1.
[0058] Table 1 Inhibition rate of P417 strain against Botrytis cinerea in confrontation test (3d, 20°C)
[0059]
[0060] The results of the confrontation test are shown in Table 1: the average antibacterial width of the plate confrontation test is 2.5m, and the radius of gray mold is only 1.13cm, while in the control group, the average radius of gray mold is 3.37cm, and the average inhibition rate is more than 71.4%. , which is a strain with s...
Embodiment 3
[0062] Preliminary Analysis of the Antagonism Mechanism of Strain P417
[0063] (1) Effect of P417 fermentation product on the growth rate of Botrytis cinerea
[0064] Use a puncher with an inner diameter of 9mm to take out the P417 bacterial block, transfer it to 100mL PDB, and shake the bacteria for 5 days at 25°C and 160rpm. The prepared spore suspension was centrifuged at 8,000 r / min for 10 min, and the supernatant was suction-filtered with a 0.22 μm microporous membrane to obtain a sterile fermentation liquid, namely the fermentation supernatant. Add the fermentation supernatant to PDA at 1%, 5%, and 10% and pour it onto a plate, connect a gray mold hyphae block with a diameter of 9mm on each plate, repeat each treatment 3 times, and perform 3 parallel experiments, culture at 20°C , Measure the colony diameter of Botrytis cinerea on 3d, 5d, and 7d and record relevant data. The test result is Figure 5 ,Table 2.
[0065] Table 2 Effect of P417 fermentation product on t...
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