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Application of folium chimonanthi nitentis tablet to preparation of drug for inhibiting skin melanophore A375 cell proliferation

A melanocyte and cell proliferation technology, which is applied in the field of preparation of waxberry leaves, can solve the problems of many impurities, backwardness, and large dosage for patients

Inactive Publication Date: 2016-03-16
济南新时代医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the application of the leaves of Waxburghii in the preparation of drugs for inhibiting the proliferation of human skin melanocytes A375 cells, and there is no report on the use of supercritical extraction in the preparation of the leaves of Waxyweed. The method of extracting volatile oil by boiling and steam distillation has a rough and backward process and many impurities, which leads to excessive dosage for patients and is inconvenient to take, which seriously affects the clinical application of this product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 1500g of waxberry leaves, add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 750min, and the supercritical extract is obtained. The supercritical extract is added to starch and 70% ethanol to make granules, dried, and pressed into tablets to make 500 tablets, each weighing 0.35g.

[0025] After testing, the content of quercetin and kaempferol in the finished product is 362 μg / tablet.

Embodiment 2

[0027] Take 1500g of waxberry leaves, add to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent. The extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 800min, and the supercritical extract is obtained. The supercritical extract is added to starch and 70% ethanol to make granules, dried, and pressed into tablets to make 500 tablets, each weighing 0.35g.

[0028] After testing, the content of quercetin and kaempferol in the finished product is 220 μg / tablet.

Embodiment 3

[0030] Take 1500g of waxberry leaves and add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 60°C, and the CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 700min, and the supercritical extract is obtained. The supercritical extract is added to starch and 70% ethanol to make granules, dried, and pressed into tablets to make 500 tablets, each weighing 0.35g.

[0031] After testing, the content of quercetin and kaempferol in the finished product is 308 μg / tablet.

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PUM

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Abstract

The invention belongs to the technical field of traditional Chinese medicine and particularly relates to application of a folium chimonanthi nitentis tablet to preparation of a drug for inhibiting skin melanophore A375 cell proliferation and a preparing method for the folium chimonanthi nitentis tablet. The folium chimonanthi nitentis tablet is prepared from 1500 g of folium chimonanthi nitentis as a bulk drug by the adoption of supercritical fluid extraction, and the content of quercetin and kaempfero is increased greatly.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of waxberry leaves in the preparation of medicines for inhibiting the proliferation of skin melanocyte A375 cells and the preparation method of waxberry leaves. Background technique [0002] The standard number of waxberry leaves is WS-11310 (ZD-1310)-2002, which is recorded in the National Compilation of Chinese Patent Medicine Standards Internal Medicine Pulmonary System (1) Volume. It is made from 1500g of waxberry leaves as a raw material medicine, and has the effects of pungent and cooling, relieving exterior, clearing away heat and detoxifying. For wind-heat cold, fever, aversion to cold, sore throat. [0003] In the prior art, there is no report on the application of the leaves of Waxburghii in the preparation of drugs for inhibiting the proliferation of human skin melanocytes A375 cells, and there is no report on the use ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K9/20A61P17/00
CPCA61K36/185A61K9/2059A61K2236/37
Inventor 杨志华李晶
Owner 济南新时代医药科技有限公司