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Application of ovR2E in curative effect of HBV infection treatment

A 1. ovr2e, protein technology, applied in the field of molecular biology, can solve only 30-50% of the problems

Inactive Publication Date: 2016-03-16
SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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Problems solved by technology

[0003] At present, IFN-α is one of the first-line drugs for the treatment of patients with chronic hepatitis B (chronichepatitisB, CHB). The response rate of IFN-α treatment for CHB is still only 30-50%, and the long-term reactivation rate is still high. Therefore, how to increase the activity of IFN-α to inhibit HBV replication and improve its antiviral treatment effect is urgent one of the clinical problems

Method used

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  • Application of ovR2E in curative effect of HBV infection treatment
  • Application of ovR2E in curative effect of HBV infection treatment
  • Application of ovR2E in curative effect of HBV infection treatment

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Embodiment Construction

[0030] The preferred embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0031] In vitro expression and purification of ovR2E protein and completion of its ligand binding experiment:

[0032] (1) Construction of prokaryotic expression vectors and protein purification of sheep ovR2E and shIFNAR-2a:

[0033] Trizol was used to extract total RNA from sheep lung fibroblast-like cells (cell number: SheepL1), and RT-PCR amplified the mRNA of extracellular domain of ovine type I interferon receptor subunit 2 (ovR2E for short), and the amplification primers were: :

[0034] ovR2ER: 5-AGACGCGGATCCTCGTATGTTGTGCCTGATCT-3

[0035] ovR2EL:5-CCGCTAGAATTCTTATGTAGCAGGTTCTGATGACT-3,

[0036] The amplified ovR2E gene fragment is the main extracellular active region of sheep IFNAR2 (amino acids 27-246) (ProcNatlAcadSciUSA.2001;98(11):6138-43), and the amino acid sequence is as follows:

[0037] MLLSQNVSAIGPLNLYPMVHIS...

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Abstract

The invention provides application of ovR2E in a curative effect of HBV infection treatment. The application of the ovR2E in the curative effect of HBV infection treatment is characterized in that the ovR2E protein is combined with hIFN-alpha to promote combination of a ligand and hIFNA-2c, and the ligand inhibition HBV replication activity is strengthened. According to the technical scheme, the ovR2E protein improves the hIFN-alpha inhibition HBV replication level, hR2a protein does not have strengthening and regulating effects on ligand inhibition HBV replication action, the ovR2E protein can be combined with the hIFN-alpha and strengthen the ligand inhibition HBV replication activity, a foundation is laid for the situation that the ovR2E protein and the hIFN-alpha are prepared into a mixture used for improving the curative effect of the hIFN-alpha on treating CHB in future, and a new approach is provided for treating the CHB.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to the application of the curative effect of ovR2E protein in the treatment of HBV infection. Background technique [0002] Hepatitis B virus (hepatitis B virus) is a DNA virus that causes acute and chronic hepatitis in humans. Today, HBV infection is a serious threat to human health. About one million people die every year from liver failure, cirrhosis and liver cancer caused by HBV infection. . [0003] At present, IFN-α is one of the first-line drugs for the treatment of patients with chronic hepatitis B (chronichepatitisB, CHB). The response rate of IFN-α treatment for CHB is still only 30-50%, and the long-term reactivation rate is still high. Therefore, how to increase the activity of IFN-α to inhibit HBV replication and improve its antiviral treatment effect is urgent One of the clinical problems. [0004] Human IFN-α (humanIFN-α, hIFN-α) combines with human type...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P31/20
Inventor 余治健邓启文陈重程航邓向斌李多云郑金鑫
Owner SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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