Microwave flow cytometer and its measuring method

Abstract

The invention provides a microwave flow cytometer, comprising a synchronous signal source, a test sensor, a contrast sensor, two microwave detectors, a differential amplifier, an A / D converter, a microprocessor, and the synchronous signal source is connected to the test sensor and the contrast sensor The excitation end, the test sensor and the control sensor are coplanar waveguides with multi-layer dielectrics embedded with conductor strips with wide ends and narrow middle. U-shaped liquid pipes are placed on the waveguide as detection channels. The output terminals of the two coplanar waveguides are connected, and the differential amplifier is connected with the microprocessor through an A / D converter. The present invention also provides a method for measuring the dielectric constant of cells by using a microwave flow cytometer, including calibration, preparation, Test and calculation; the invention reduces the error caused by the change of the dielectric constant of the culture medium, avoids the use of expensive equipment such as vector network analyzers, adopts the modular design concept, and the functions of each module are clear, which is easy for later maintenance and repair work.

Description

technical field [0001] The invention relates to a microwave flow cytometer for reversing the dielectric constant of cells in a suspension by using microwave network scattering parameters and a method for measuring the dielectric constant of cells by using the cytometer, belonging to the field of microwave measurement. Background technique [0002] Flow cytometer is an advanced detection instrument developed in the early 1970s for automatic analysis and sorting of cells. It can quickly measure, store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out specified cell subpopulations according to the pre-selected parameter range. Flow cytometer mainly consists of four parts, they are: flow chamber and liquid flow system; laser source and optical system; photoelectric tube and detection system; computer and analysis system. The general working principle of flow cytometer: flow cytometer...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of complex, expensive and bulky traditional flow cytometer technology; starting from the field of microwave measurement technology, by directly measuring the scattering parameters of the microwave network, inverting the cell dielectric constant, and then analyzing the cell structure purpose of nature

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