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Ruminant animal-origin feedstuff identification method based on lipid Raman spectrums

A technology of animal-derived feed and Raman spectroscopy is applied in the field of identification and analysis of animal-derived feed raw materials using Raman spectroscopy, which can solve problems such as low detection accuracy, meet analysis requirements, effectively identify analysis, and implement feasible The effect of sustainable development and recycling

Inactive Publication Date: 2016-03-23
CHINA AGRI UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

The existing near-infrared spectroscopy technology is based on the detection of raw materials. It is a rapid detection method, but the detection accuracy is relatively low, and it also has certain limitations in the identification and analysis of species.

Method used

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  • Ruminant animal-origin feedstuff identification method based on lipid Raman spectrums
  • Ruminant animal-origin feedstuff identification method based on lipid Raman spectrums
  • Ruminant animal-origin feedstuff identification method based on lipid Raman spectrums

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Embodiment 1

[0037] 1) Collection and preparation of research samples

[0038] The research samples are 70 animal-derived feeds of known animal species, including products collected from domestic and foreign protein feed enterprises and self-made samples by relevant quality inspection departments. The samples cover terrestrial animal meat meal, bone meal, meat and bone meal and fishmeal, etc., including 21 samples from ruminants (11 from cattle, 9 from sheep), 32 samples from non-ruminants (11 from pigs, 13 from chickens) and 26 samples from fish.

[0039] All research samples were pulverized by a cyclone mill and passed through a 1.0 mm sieve.

[0040] 2) Lipid sample extraction

[0041] The lipid samples of the above-mentioned research samples were extracted by the Soxhlet extraction method using an automatic fat analyzer (SoxtecTM2050, FOSS Company, Denmark).

[0042] 3) Raman spectrum information data of lipid samples

[0043] BrukerOpticsFT-RamanVERTEX70v (Bruker, Germany) was used...

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Abstract

The invention provides a ruminant animal-origin feedstuff identification method based on lipid Raman spectrums. The ruminant animal-origin feedstuff identification method includes the steps that animal-origin feedstuff with known origins and of different species are collected; lipid samples are extracted; Raman spectrum information data of the lipid samples are collected, and the spectrum range is 400-3600 cm<-1>; a distinguishing and analyzing model of the ruminant animal-origin feedstuff is built; the built distinguishing and analyzing model is evaluated; lipid samples of the animal-origin feedstuff to be detected are extracted, and the Raman spectrum information data are collected and input into the distinguishing and analyzing model for species identification. The Raman spectrums of the lipid samples are directly collected, spectrum information and species origin information are correlated, the ruminant animal-origin feedstuff can be identified and analyzed fast and effectively, the analyzing requirement for species identification of the ruminant animal-origin feedstuff of feed quality safety supervision in China is met, and it can be ensured that while 'bovine spongiform encephalopathy' is efficiently prevented, and sustainable development and cyclic utilization of the feed industry are effectively implemented.

Description

technical field [0001] The invention belongs to the field of identification of animal-derived feed, in particular to a method for identifying and analyzing animal-derived feed raw materials by using Raman spectroscopy. Background technique [0002] Mad Cow Disease (Bovine Spongiform Encephalopathy) is a zoonotic disease whose pathogenic prion can be transmitted through the food chain. Meat and bone meal is an animal-derived protein feed made from non-edible tissues after slaughtering livestock and subjected to high-temperature treatment. It has the characteristics of rich protein content and has been widely used in the feed industry. Studies have shown that mad cow disease is caused by feeding ruminants with meat and bone meal from sick cattle, sheep and other animals. In order to prevent the spread of mad cow disease, countries around the world have formulated relevant laws and regulations to restrict or prohibit the use of animal-derived feed such as meat and bone meal. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 刘贤高菲徐凌芝杨增玲韩鲁佳
Owner CHINA AGRI UNIV
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