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Biosensor used for detecting DNA (deoxyribonucleic acid) bases and constructed on basis of working electrode modified by piperazine modified reducing graphene oxide

A biosensor and working electrode technology, applied in the field of DNA base detection, can solve the problems of increasing the incidence of gout diseases, etc., and achieve the effect of wide linear detection range, high sensitivity and low detection limit

Inactive Publication Date: 2016-03-23
HENAN UNIVERSITY
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  • Abstract
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  • Application Information

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Problems solved by technology

However, a large amount of meat intake will increase the incidence of some diseases such as gout and hyperuria

Method used

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  • Biosensor used for detecting DNA (deoxyribonucleic acid) bases and constructed on basis of working electrode modified by piperazine modified reducing graphene oxide
  • Biosensor used for detecting DNA (deoxyribonucleic acid) bases and constructed on basis of working electrode modified by piperazine modified reducing graphene oxide
  • Biosensor used for detecting DNA (deoxyribonucleic acid) bases and constructed on basis of working electrode modified by piperazine modified reducing graphene oxide

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Embodiment 1

[0025] Such as figure 1 As shown, a biosensor for detecting DNA bases is constructed by using piperazine-modified reduced graphene oxide-modified working electrode. The biosensor includes a working electrode, an auxiliary electrode (platinum electrode) and a reference electrode (Ag / AgCl electrode), wherein the working electrode is a piperazine-modified reduced graphene oxide-modified glassy carbon electrode, which is obtained through the following steps:

[0026] 1) After mixing equal volumes (5 μL) of 0.5% chitosan solution and 4 mg / ml piperazine-modified reduced graphene oxide solution, ultrasonically disperse for about 0.5 h, then stand for about 2 h to obtain nanocomposites, and re-sonicate , After vortexing to disperse evenly, use a pipette to absorb 4μL and drop it slowly and evenly onto the surface of a clean glassy carbon electrode, and dry to form a film (to ensure no air bubbles, no side leakage, and no pollution, it can be placed in a desiccator overnight for dryin...

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Abstract

The invention discloses a biosensor used for detecting DNA (deoxyribonucleic acid) bases and constructed on the basis of a working electrode modified by piperazine modified reducing graphene oxide. The biosensor comprises the working electrode, an auxiliary electrode and a reference electrode, wherein the working electrode adopts a glassy carbon electrode modified by piperazine modified reducing graphene oxide and is obtained through the following steps: 1) a chitosan solution and a piperazine modified reducing graphene oxide solution are mixed in an equal volume manner, a mixture is subjected to ultrasonic dispersion for 0.5-1 h and left to stand for 1-3 h, and a composite material is obtained, subjected to ultrasonic oscillation and vortex dispersion and added dropwise to the clean glassy carbon electrode surface and air-dried into a film; 2) a Nafion solution is added dropwise to the glassy carbon electrode film surface obtained in the step 1) and air-dried into a film, and the working electrode is obtained. The biosensor can realize separate or simultaneous detection of the DNA bases and has the advantages of wider linear detection range, lower detection limit, higher sensitivity, few interference reactions and mild reaction conditions.

Description

technical field [0001] The invention belongs to the technical field of DNA base detection, and in particular relates to a novel method for detecting DNA bases by a biosensor constructed by using piperazine-modified reducing graphene oxide modified electrodes. Background technique [0002] Base is the main component of nucleic acid molecules. Nucleic acid analysis is of great significance in biochemistry and molecular biology research, and is widely used in clinical detection of infectious diseases, epidemics, tumors and genetic diseases, and environmental monitoring. Electrochemistry has been widely recognized by researchers at home and abroad because of its simple operation, rapidity, and sensitivity. The use of electrochemical methods for nucleic acid analysis has become a research hotspot at home and abroad. [0003] Adenine (A), thymine (T), guanine (G) and cytosine (C) in nucleic acid molecules play important roles in storing genetic information and in protein biosynthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/308G01N27/3277
Inventor 洪军杨晓璐肖保林
Owner HENAN UNIVERSITY
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