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Electrochemical sensing method for accurately detecting trace protein molecules

A protein molecule and sensing method technology, applied in the field of electrochemical sensing for the precise detection of trace protein molecules, can solve the problems that the detection results are easily interfered by other coexisting substances, the electrode preparation process is complicated, and the reliability is difficult to achieve. Achieve the effect of enhancing chemical stability, improving detection specificity, and ensuring detection accuracy

Pending Publication Date: 2021-10-29
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing electrochemical detection methods rely on the modification of biomolecules on the electrode surface. The electrode preparation process is complicated, the chemical stability is poor, and the detection results are easily interfered by other coexisting substances. Therefore, the reliability is still difficult to meet the requirements of practical applications.

Method used

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  • Electrochemical sensing method for accurately detecting trace protein molecules
  • Electrochemical sensing method for accurately detecting trace protein molecules
  • Electrochemical sensing method for accurately detecting trace protein molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A kind of ovalbumin (OVA) electrochemical sensing method based on biocoupling and magnetic molecular imprinting technology is:

[0048] Preparation of Magnetic Molecularly Imprinted Nanoparticles of S1 OVA Protein

[0049] 1. Aminated magnetic Fe 3 o 4 Preparation of nanoparticles: First, add 2.0 g of ferric chloride hexahydrate, 13.0 g of 1,6-hexanediamine and 4.0 g of anhydrous sodium acetate in a beaker; secondly, add 60 mL of ethylene glycol to dissolve, and use a magnetic stirrer Stir until the solution is yellow and free of precipitates; then, pour the mixture into a polytetrafluoroethylene high-pressure reactor and react at 200°C for 6 h; finally, after the reaction is completed, cool to room temperature, wash with water and ethanol three times, and Dry under vacuum at 50°C to prepare aminated magnetic Fe 3 o 4 nanoparticles;

[0050] 2. Boric acid modified magnetic Fe 3 o 4 Preparation of nanoparticles: First, take 0.40 g of aminated magnetic Fe 3 o 4 N...

Embodiment 2

[0060] The steps of a carcinoembryonic antigen (CEA) electrochemical sensing method based on biocoupling and magnetic molecular imprinting technology are:

[0061] Preparation of Magnetic Molecularly Imprinted Nanoparticles of S1 CEA Protein

[0062] With reference to the S1 step method in Example 1, prepare aminated magnetic Fe 3 o 4 Nanoparticles, boric acid modified magnetic Fe 3 o 4 Oriented Imprinting of Magnetic Fe on the Surface of Nanoparticles, CEA Proteins 3 o 4 @SiO 2 - MIP nanoparticles.

[0063] Enrichment of S2 CEA protein: referring to the S2 step method in Example 1, the magnetic Fe imprinted by CEA 3 o 4 @SiO 2 -MIP was added to the solution containing CEA protein, incubated with shaking at room temperature to obtain Fe 3 o 4 @SiO 2 - CEA magnetic nanoparticles;

[0064] S3 electrical signal conversion: with reference to the S3 step method in Example 1, electroactive tyrosine is biocoupled to Fe 3 o 4 @SiO 2 -CEA magnetic nanoparticle surface, ...

Embodiment 3

[0070] The steps of an electrochemical sensing method for alpha-fetoprotein (AFP) based on metal coordination and magnetic molecular imprinting technology are:

[0071] Preparation of Magnetic Molecularly Imprinted Nanoparticles of S1 AFP Protein

[0072] With reference to the S1 step method in Example 1, prepare aminated magnetic Fe 3 o 4 Nanoparticles, boric acid modified magnetic Fe 3 o 4 Magnetic Fe for Oriented Imprinting on the Surface of Nanoparticles, AFP Proteins 3 o 4 @SiO 2 - MIP nanoparticles.

[0073] Enrichment of S2 AFP protein: referring to the S2 step method in Example 1, the magnetic Fe imprinted by AFP 3 o 4 @SiO 2 -MIP was added to the solution containing AFP protein, incubated with shaking at room temperature to obtain Fe 3 o 4 @SiO 2 - AFP magnetic nanoparticles;

[0074] S3 electrical signal conversion: the Fe obtained in step S2 3 o 4 @SiO 2 - AFP was dispersed in 190 μL PBS, and 10 μL of Hg with a concentration of 10 mmol / L was added 2...

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Abstract

The invention relates to the technical field of rapid detection of disease markers, in particular to an electrochemical sensing method for accurately detecting trace protein molecules. According to the method, based on a magnetic molecular imprinting and electric signal conversion strategy, an amperometric electrochemical sensing method for sensitive detection of trace protein molecules is constructed, wherein specific enrichment and separation of protein molecules are guaranteed through a magnetic molecular imprinting technology, electroactive molecules or ionic protein molecules are combined through biological coupling or metal coordination so as to perform electric signal conversion, and finally, the content of the electroactive substance is determined through an electrochemical method so as to realize the electrochemical analysis and detection of the non-electroactive protein molecules. According to the invention, the magnetic molecular imprinting technology and the electrochemical sensing technology are comprehensively utilized, high-sensitivity detection of various trace protein molecules in a complex biological sample can be simply, specifically and accurately achieved at low cost, and the method is suitable for clinical examination and early diagnosis of serious diseases.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and separation, and in particular relates to an electrochemical sensing method for precise detection of trace protein molecules. Background technique [0002] As an important component of human cells and tissues, protein is involved in almost all life processes and is closely related to the occurrence and development of various diseases. Proteins have been widely used as disease markers in clinical diagnosis and condition assessment of major diseases such as cancer, cardiovascular and infectious diseases. Therefore, carrying out accurate protein detection research has very important scientific research value and clinical application value in the early diagnosis, treatment, prevention and control of major diseases. [0003] Existing protein detection methods mainly rely on mass spectrometry, chromatography, chromatography-mass spectrometry, capillary electrophoresis, enzyme-linked...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/333G01N27/30G01N27/48
CPCG01N27/3278G01N27/3277G01N27/333G01N27/308G01N27/48
Inventor 李天保张宝金韦霜霜孙丽丽王进义
Owner NORTHWEST A & F UNIV
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