Disinfection method of grape explant in tissue culture

Abstract

The invention discloses a disinfection method of a grape explant in tissue culture. The disinfection method comprises the following steps: a grape stem section is washed with sterile water for 3-5 times and then washed with 75% alcohol for 30-50 seconds, and a grape stem section after alcohol washing is obtained; the grape stem section is washed with the sterile water for 3-5 times and soaked in 800-1,000 times of the 84 disinfectant for 2-4 minutes, and the grape stem section after 84 disinfectant soaking is obtained; the grape stem section is washed with the sterile water for 3-5 times and soaked in 0.1% mercuric chloride for 5-8 minutes, and the grape stem section after mercuric chloride soaking is obtained; the grape stem section is washed with the sterile water for 3-5 times, and disinfection is finished for inoculation. The disinfection method has the following benefits: after the disinfection method of the grape explant in tissue culture is adopted for disinfection, the explant is inoculated to a medium for culture, the mould generation rate after rooting and leafing can be reduced by more than 60%, and accordingly, the method can effectively reduce mould contamination of the explant after inoculation.

Description

technical field [0001] The invention relates to the field of disinfection methods for plant tissue explants, in particular to a disinfection method for grape tissue culture explants. Background technique [0002] The mass collection of grape explants is in July every year. If there is a lot of rain, the disease will be very serious. Even if there is no mold symptom on the grape leaves in the field, the plant will also carry bacteria recessively. After the explants are inoculated into the medium 10- After 15 days, after the explants have taken root and expanded leaves, due to the division of plant cells, internal mold pollution occurs during plant growth. It is difficult to disinfect thoroughly with the current commonly used disinfection methods (generally explants are inoculated and cultured, and mold contamination occurs within 3 days after inoculation). Contents of the invention [0003] Purpose of the present invention is exactly at the defective in the above-mentioned...

AI Technical Summary

Problems solved by technology

It is difficult to thoroughly disinfect with the current commonly used disinfection methods (generally, the explants are inoculated and cultured, and mold contamination occurs within 3 days after inoculation)