Microbial growth promoter specially used for ecological planting of wild rice stems and preparation method thereof

A growth promoter and ecological planting technology, applied in the field of microorganisms, can solve the problems of low yield of water jasmine, deterioration of soil and soil quality, and compaction.

Inactive Publication Date: 2016-04-06
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the traditional planting method of Zizania, the problems of soil agglomeration and compaction are extremely serious due to the ext

Method used

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preparation example Construction

[0030] The invention provides a method for preparing a microbial growth promoter specially used for the ecological cultivation of Zizania zizania, which is characterized in that it comprises:

[0031] 1) Preliminary screening out Pseudomonas, Azotobacter syringae, Bacillus subtilis, Methylomonas and Streptomyces from Zizania japonica planting soil, and further screening to obtain strains with fast growth rates;

[0032] 2) Mixing and culturing the strains obtained in step 1) in a liquid medium to obtain a mixed bacterial liquid;

[0033] 3) performing solid expansion culture on the mixed bacterial solution obtained in step 2) in the solid expansion medium to obtain the initial fermentation product;

[0034] 4) drying the initial fermentation product obtained in step 3), adding a matrix carrier, sieving, and packaging to obtain the microbial growth promoter.

[0035] In one embodiment of the present invention, the specific steps of the preliminary screening are: soaking the Zi...

Embodiment 1

[0054] A preparation method of a microbial growth promoter specially used for the ecological cultivation of Zizania zizania, specifically:

[0055] Step 1: Breeding of Fermentation Strains

[0056] a. Bacterial strain screening: choose well-growing planting soil, add 10 times the weight of the soil to the sterile water and soak for 24 hours, use photosynthetic bacteria medium to screen out Rhodopseudomonas in the leaching solution, and use nitrogen-fixing medium to screen the original Brown Azotobacter, use beef extract peptone agar medium to screen Bacillus subtilis and Methylomonas, use potato sucrose agar medium to screen Streptomyces;

[0057] b. Shake flask count screening: the above strains that were screened were diluted to 10% with 0.9% normal saline. -5 The final bacterial suspension was cultured upside down on a nutrient agar plate at 35°C for 48 hours, and the colony with a fast growth rate was selected for a shake flask experiment to observe the growth rate of the...

Embodiment 2

[0074] A method for preparing a microbial growth promoter specially used for the ecological cultivation of Zizania zizania, specifically:

[0075] Step 1: Breeding of Fermentation Strains

[0076] a. Bacteria screening: select well-growing planting soil, add 10 times the weight of the soil to the sterile water and soak for 26 hours, use photosynthetic bacteria medium to screen out Rhodopseudomonas in the leaching solution, and use nitrogen-fixing medium to screen the original Brown Azotobacter, use beef extract peptone agar medium to screen Bacillus subtilis and Methylomonas, use potato sucrose agar medium to screen Streptomyces;

[0077] b. Shake flask counting and screening: Dilute the above strains with a concentration of 0.9% normal saline to 10-7 and place the bacterial suspension on a nutrient agar plate at 35°C for 48 hours, and select colonies with fast growth Conduct shake flask experiments, observe the growth rate of the strains, and screen the strains with a quanti...

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Abstract

The invention provides a preparation method of a microbial growth promoter specially used for ecological planting of wild rice stems. The preparation method comprises the following steps: 1) preliminarily screening pseudomonas, azotobacter chroococcum, bacillus subtilis, methylomonas and streptomycete respectively from wild rice stem planting soil and respectively obtaining strains with high growth rates through further screening; 2) carrying out mixed culture on the strains obtained in the step 1) in a liquid culture medium to obtain mixed bacterium liquid; 3) carrying out solid enlarge culture on the mixed bacterium liquid in a solid enlarge culture medium to obtain an initial fermented product; and 4) drying the initial fermented product, adding matrix carriers and carrying out sieving and subpackaging, thus obtaining the microbial growth promoter. The inventors of the application improve existing wild rice stem planting methods and design the planting methods and fertilizer use according to the law of requirements of wild rice stems for fertilizers in the growth process, thus obtaining higher yield and high-quality fruit and increasing the economic benefits of planting.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a microbial growth promoter, in particular to a microbial growth promoter specially used for the ecological planting of wild rice stems and a preparation method thereof. Background technique [0002] Zizania is a vegetatively propagated aquatic perennial gramineous crop, commonly known as: Zizania bamboo shoot, Zizania melon, its fleshy stem contains 4% sugar, 1.5% protein, and is rich in multivitamins, so it is deeply loved by consumers. Zizania is native to China and Southeast Asia. It is a relatively common aquatic vegetable. It is a spindle-shaped fat part formed by the tender stems of the Gramineae plant, which is stimulated by the fungus Ustilago smut. At present, only China and Vietnam cultivate Zizania as a vegetable, and China is the earliest. Wild rice stems are distributed throughout the north and south of my country, and are planted in Hebei, Jiangsu, Zhejiang, Anhui, J...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCC05F5/002C05F11/08C05F11/00
Inventor 江瀚陆瑛
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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