Bacillus aquimaris L-60 capable of effectively prompting growth of crops and application thereof
A seawater bacillus, plant growth-promoting technology, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve obvious growth-promoting effect, promote crop growth, and stabilize growth-promoting ability
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Embodiment 1
[0026] Embodiment 1, isolation and identification of functional strains
[0027] Put 5g of potato roots cut into small pieces into a triangular flask filled with 45ml of sterile water, vibrate at 30°C for 30min (170r / min) to make a rhizosphere soil suspension, put the suspension in a water bath at 80°C for 20min, and then Equipped with 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 soil dilution. Take 0.1mL rhizospheric soil suspension with different dilution gradients, put it on LB plate and spread it, place it in an incubator at 30°C for 24 hours, observe the growth of colonies on the plate, select different colonies for purification, and inoculate the purified strains on the slant for culture and stored in a 4°C refrigerator until use.
[0028] From the isolated bacteria, the functional strains capable of producing IAA were screened. The screening method was: adding 2 mg / mL L-tryptophan mother liquor that had been filtered and sterilized into 3 mL liquid LB medium to make the fina...
Embodiment 2
[0031] Embodiment 2, the generation of functional bacteria filling bacteria suspension
[0032] The bacterial strain L-60 (CGMCCNo.10285) was inoculated into the PDA culture medium for liquid fermentation production. The conditions for fermentation production were: pH 7.0, fermentation temperature range 30°C, stirring speed 170 rpm, and fermentation time 48h. Bacteria content in fermentation broth ≥ 1×10 10 Individuals / mL; The bacteria filling solution is the bacterial suspension obtained by resuspending the bacteria with an equal volume of 0.9% normal saline after centrifuging the fermentation broth at 6000r / min for 8 minutes, or directly using the fermentation broth as the bacteria filling solution.
[0033] The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: Peel 200g of potatoes and cut into small pieces and put them in water to boil, boil for 30min after boiling, add 20g of ordinary sucrose to the filtrat...
Embodiment 3
[0034] Embodiment 3, potted plant growth-promoting effect test
[0035] 3.1 Application effect of growth-promoting biofertilizer on corn (first season, second season)
[0036] The growth-promoting pot experiment was carried out in the greenhouse of the National Organic Fertilizer Engineering Technology Research Center (Yixing). The corn variety used was Suyu 29. The corn seeds were soaked in normal temperature water for 4 hours, placed on moist gauze in a 30°C incubator Accelerate germination in the middle for 2-3 days. After more than 75% of the seeds germinate, select the dewy seeds and move them to the seedling tray for seedling cultivation. After 2 true leaves grow, select the seedlings with the same growth and transplant them into the pot. Two treatments were set up in the experiment: 1) CK, only application of decomposed chicken manure; 2) treatment of L-60, application of decomposed chicken manure and bacterial suspension of L-60. Each handles 8 pots, each pot contains...
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