Bacillus aquimaris L-60 capable of effectively prompting growth of crops and application thereof

A seawater bacillus, plant growth-promoting technology, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve obvious growth-promoting effect, promote crop growth, and stabilize growth-promoting ability

Inactive Publication Date: 2016-04-06
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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At present, there is no related report that Bacillu

Method used

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  • Bacillus aquimaris L-60 capable of effectively prompting growth of crops and application thereof
  • Bacillus aquimaris L-60 capable of effectively prompting growth of crops and application thereof
  • Bacillus aquimaris L-60 capable of effectively prompting growth of crops and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, isolation and identification of functional strains

[0027] Put 5g of potato roots cut into small pieces into a triangular flask filled with 45ml of sterile water, vibrate at 30°C for 30min (170r / min) to make a rhizosphere soil suspension, put the suspension in a water bath at 80°C for 20min, and then Equipped with 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 soil dilution. Take 0.1mL rhizospheric soil suspension with different dilution gradients, put it on LB plate and spread it, place it in an incubator at 30°C for 24 hours, observe the growth of colonies on the plate, select different colonies for purification, and inoculate the purified strains on the slant for culture and stored in a 4°C refrigerator until use.

[0028] From the isolated bacteria, the functional strains capable of producing IAA were screened. The screening method was: adding 2 mg / mL L-tryptophan mother liquor that had been filtered and sterilized into 3 mL liquid LB medium to make the fina...

Embodiment 2

[0031] Embodiment 2, the generation of functional bacteria filling bacteria suspension

[0032] The bacterial strain L-60 (CGMCCNo.10285) was inoculated into the PDA culture medium for liquid fermentation production. The conditions for fermentation production were: pH 7.0, fermentation temperature range 30°C, stirring speed 170 rpm, and fermentation time 48h. Bacteria content in fermentation broth ≥ 1×10 10 Individuals / mL; The bacteria filling solution is the bacterial suspension obtained by resuspending the bacteria with an equal volume of 0.9% normal saline after centrifuging the fermentation broth at 6000r / min for 8 minutes, or directly using the fermentation broth as the bacteria filling solution.

[0033] The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: Peel 200g of potatoes and cut into small pieces and put them in water to boil, boil for 30min after boiling, add 20g of ordinary sucrose to the filtrat...

Embodiment 3

[0034] Embodiment 3, potted plant growth-promoting effect test

[0035] 3.1 Application effect of growth-promoting biofertilizer on corn (first season, second season)

[0036] The growth-promoting pot experiment was carried out in the greenhouse of the National Organic Fertilizer Engineering Technology Research Center (Yixing). The corn variety used was Suyu 29. The corn seeds were soaked in normal temperature water for 4 hours, placed on moist gauze in a 30°C incubator Accelerate germination in the middle for 2-3 days. After more than 75% of the seeds germinate, select the dewy seeds and move them to the seedling tray for seedling cultivation. After 2 true leaves grow, select the seedlings with the same growth and transplant them into the pot. Two treatments were set up in the experiment: 1) CK, only application of decomposed chicken manure; 2) treatment of L-60, application of decomposed chicken manure and bacterial suspension of L-60. Each handles 8 pots, each pot contains...

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Abstract

The invention provides bacillus aquimaris L-60 capable of effectively prompting the growth of crops and application thereof. The strain is separated from potato rhizosphere, and is classified into bacillus aquimaris based on physiological-biochemical characteristics and 16S rDNA (ribosome Deoxyribose Nucleic Acid) analysis. As proved by a high performance liquid chromatography analysis result, the strain L-60 has a high indole Acetic acid (IAA) producing capability. As proved by a pot experiment result, a growth-promoting bio-fertilizer researched on the basis of the strain can remarkably promote the growth of corn, cucumber and tomato plants.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a strain of sea bacillus L-60 which can effectively promote crop growth and its application. Background technique [0002] Plant growth-promoting rhizobacteria (Plant Growth-promoting Rhizobacteria, PGPR) refers to microorganisms that exist on the surface of the plant rhizosphere and in the soil microenvironment around the root system, and can significantly promote plant growth through direct or indirect means (Ahmadetal, 2008). Usually the most significant growth-promoting ability of rhizosphere growth-promoting bacteria is the ability to secrete phytohormones. Indole-3-acetic acid (IAA), which has been studied more, promotes plant growth by promoting cell elongation and mitosis (Glicketal ,1998), and IAA can dissolve insoluble phosphorus in the soil and improve the absorption of phosphorus in the soil by crops (Li Haizong et al., 2014). Obtaining high-eff...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 沈其荣李荣刘小玉张瑞福高旭王甜甜
Owner NANJING AGRICULTURAL UNIVERSITY
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