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Method for detecting content of various components in pills for treating kidney-yang deficiency

A detection method and technology of Yougui Pills, which are applied in the directions of measuring devices, material separation, and analysis of materials, etc., can solve the problems of one-sided quality evaluation of Yougui Pills, limited coverage, and unclear chemical composition of Yougui Pills.

Active Publication Date: 2016-04-13
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Application Information

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Problems solved by technology

However, the above studies only selected one active ingredient in Yougui Pills for determination or qualitative analysis, and the coverage is relatively limited. The other chemical components in Yougui Pills are still unclear, and the quality evaluation applied to Yougui Pills is inevitably relatively one-sided.

Method used

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  • Method for detecting content of various components in pills for treating kidney-yang deficiency
  • Method for detecting content of various components in pills for treating kidney-yang deficiency
  • Method for detecting content of various components in pills for treating kidney-yang deficiency

Examples

Experimental program
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Embodiment 1

[0103] Embodiment 1, measure 16 kinds of determination components in Yougui Pill

[0104] 1) Preparation of standard solution: Accurately weigh appropriate amounts of 16 standard products, dissolve them in dimethyl sulfoxide (DMSO) respectively, and prepare separate standard stock solutions with a concentration of 1.00 mg / mL;

[0105] Accurately measure the appropriate amount of the above-mentioned standard stock solution (measured volume depends on the mass spectrometry response of each compound), place in the same 1.5mLEP tube, add methanol to dilute to obtain a mixed standard stock solution, wherein the concentration of each compound is as follows: Neoaconitine 200ng / mL, Cornus glucoside 500ng / mL, Rehmanniaside A200ng / mL, Fuziling 200ng / mL, Songguolin 200ng / mL, Hyperoside 500ng / mL, Benzoyl aconitine 200ng / mL, morroniside 1000ng / mL, benzoyl aconitine 200ng / mL, rehinoside D500ng / mL, benzoyl aconitine 500ng / mL, nyolin 500ng / mL, loganin 1000ng / mL, aconitine 100ng / mL, swertig...

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Abstract

The invention discloses a method for detecting the content of various components in pills for treating kidney-yang deficiency. The method includes the following steps that firstly, the pills for treating kidney-yang deficiency are extracted in an ultrasonic mode through methyl alcohol and then filtered through a filtering membrane, primary filtering liquid is abandoned, and subsequent filtering liquid serves as a test product solution; secondly, an internal label solution is added into the test product solution to serve as a sample to be tested; thirdly, the sample to be tested is detected through efficient liquid chromatography and electrospray tandem mass spectrometry, and the content of any component in the pills for treating kidney-yang deficiency is obtained. The built LC-ESI-MS / MS (high-performance liquid chromatography coupled with mass spectrometry) detection method has the advantages of being high in sensitivity and specificity, can be used for quantitatively measuring 16 components in 20 bathes of pills from different sources and for treating kidney-yang deficiency in the market, and is wide in coverage in order to build a systematic and comprehensive quality assessment method for the pills for treating kidney-yang deficiency.

Description

technical field [0001] The invention relates to a method for detecting the contents of various components in Yougui Pills, belonging to the field of component detection. Background technique [0002] Youguiwan comes from "Jingyue Quanshu". Its composition and preparation method are "Dahuai rehmannia glutinosa eight liang, yam (fried) four liang, dogwood (slightly fried) three liang, wolfberry (slightly fried) four liang, antler gum (fried) Beads) 4 liang, Cuscuta (made) 4 liang, Eucommia ulmoides (stir-fried with ginger) 4 liang, angelica 3 liang (do not use for loose stools), cinnamon 2 liang (gradually increase to 4 liang), made aconite 2 liang (gradually can Add to five or six liang). First, steam the rehmannia glutinosa and paste it with pestle, add refined honey to make pills, which are as big as phoenix seeds." Yougui Pills are widely used clinically to treat the syndromes of kidney-yang deficiency, and have definite curative effects. [0003] At present, there are f...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 王蕾刘浩龙仇峰赵晖邹海艳杨鑫伟
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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