Breeding method for rape restorer with leaf margin divided marker character
A restorer and cleavage technology, applied in the fields of application, botanical equipment and methods, and plant genetic improvement, can solve problems such as difficult identification of population purity and true and false hybrids, unobvious marker traits of rapeseed, and susceptibility to environmental influences, etc. , to achieve the effect of easily identifying restorer lines and sterile lines, ensuring seed purity and yield, and ensuring seed purity
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Embodiment 1
[0041] The rape restorer line selection method with leaf edge full crack mark character described in this implementation, it specifically comprises the following steps:
[0042] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:
[0043] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;
[0044] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and BC was obtained by backcrossing the new Brassica napus line (RS013-008) AACC as the male parent 1 F backcross group;
[0045] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as the female par...
Embodiment 2
[0054] The breeding process of the rape restorer line breeding method with the leaf edge full crack marker trait described in this implementation is as follows figure 1 As shown, it specifically includes the following steps:
[0055] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:
[0056] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;
[0057] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and BC was obtained by backcrossing the new Brassica napus line (RS013-008) AACC as the male parent 1 F backcross group;
[0058] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait...
Embodiment 3
[0065] The method for selecting a restorer line of rapeseed with leaf edge full-crack marker traits described in this implementation, specifically comprises the following steps:
[0066] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: image 3 As shown, it specifically includes the following steps:
[0067] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;
[0068] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and BC was obtained by backcrossing the new Brassica napus line (RS013-008) AACC as the male parent 1 F backcross group;
[0069] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as the fe...
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