Breeding method for rape restorer with leaf margin divided marker character

A restorer and cleavage technology, applied in the fields of application, botanical equipment and methods, and plant genetic improvement, can solve problems such as difficult identification of population purity and true and false hybrids, unobvious marker traits of rapeseed, and susceptibility to environmental influences, etc. , to achieve the effect of easily identifying restorer lines and sterile lines, ensuring seed purity and yield, and ensuring seed purity

Active Publication Date: 2016-04-20
江西省农业科学院作物研究所
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the technical problem to be solved by the present invention is to overcome the inconspicuous and unstable marker traits of rapeseed in the prior art, which is easily affected by the environment, and it is not easy to distinguish the purity of the population and the true and false hybrids, thereby

Method used

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  • Breeding method for rape restorer with leaf margin divided marker character
  • Breeding method for rape restorer with leaf margin divided marker character
  • Breeding method for rape restorer with leaf margin divided marker character

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The rape restorer line selection method with leaf edge full crack mark character described in this implementation, it specifically comprises the following steps:

[0042] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:

[0043] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;

[0044] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and BC was obtained by backcrossing the new Brassica napus line (RS013-008) AACC as the male parent 1 F backcross group;

[0045] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as the female par...

Embodiment 2

[0054] The breeding process of the rape restorer line breeding method with the leaf edge full crack marker trait described in this implementation is as follows figure 1 As shown, it specifically includes the following steps:

[0055] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:

[0056] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;

[0057] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and BC was obtained by backcrossing the new Brassica napus line (RS013-008) AACC as the male parent 1 F backcross group;

[0058] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait...

Embodiment 3

[0065] The method for selecting a restorer line of rapeseed with leaf edge full-crack marker traits described in this implementation, specifically comprises the following steps:

[0066] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: image 3 As shown, it specifically includes the following steps:

[0067] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;

[0068] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and BC was obtained by backcrossing the new Brassica napus line (RS013-008) AACC as the male parent 1 F backcross group;

[0069] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as the fe...

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Abstract

The invention discloses a breeding method for a rape restorer with a leaf margin divided marker character. The breeding method comprises: a plant with the leaf margin divided marker character is distantly hybridized with rapes, a stable rape inbred line with the leaf margin divided marker character is obtained and taken as a male parent, a rape restorer producing excellent is taken as a female parent, hybridization and breeding are performed to obtain a rape restorer with the leaf margin divided marker character, and the rape restorer with the leaf margin divided marker character is obtained, the leaf margin divided marker character has strong specificity and is controlled by a pair of dominant major genes, the character is stable and is not influenced by environmental conditions, the leaf margin divided marker character is relatively deep and is easy to recognize, and the leaf margin divided marker character can be found early and can be recognized as long as a seedling true leaf appears, so that all of plants without leaf margin divided markers can be conveniently recognized and removed in a restorer breeding process, other seeds are prevented from being mixed, and seeds of the restorer can be guaranteed.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and in particular relates to a restorer line of rapeseed with the trait of fully-cracked leaf edge markers, a breeding method and application thereof. Background technique [0002] At present, more than 80% of my country's rapeseed production uses hybrid varieties. Hybrid varieties include three-line hybrids and two-line hybrids. [0003] The production of three-line hybrids includes restorer breeding, maintainer breeding, restorer breeding and hybrid seed production. The specific process is: the seeds of the restorer line are isolated and propagated, and the maintainer line is propagated in isolation; the restorer line is hybridized by the restorer line and the maintainer line; the hybrid for field production is obtained by the hybrid seed production of the restorer line and the restorer line. [0004] The production of two-line hybrids includes: breeding of restorer lines, breeding of r...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 戴兴临汤洁涂玉琴涂伟凤张南峰张弢王丽钦
Owner 江西省农业科学院作物研究所
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