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Method for rapid production of white and seedlings

A kind of seedling and rapid technology, applied in the biological field, can solve the problems of limited white and clinical medication, susceptibility to pathogenic bacteria, slow growth of sterile test tube seedlings, etc., achieve broad application prospects and economic value, avoid complicated operation process, and be easy to store and transport effects

Active Publication Date: 2018-07-03
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional rhizome ramets have slow propagation speed, long growth cycle, low efficiency, high cost of seedlings, and it is difficult to meet the needs of large-scale cultivation; while sterile test-tube seedlings produced by tissue culture grow slowly, have low transplanting survival rate, and are resistant to stress. Poor sex, susceptible to pathogenic bacteria
Judging from the current situation, the difficulty in reproduction of white and white has not been fundamentally resolved, resulting in the near exhaustion of wild white and wild resources, and the market is in short supply, which seriously limits the demand for clinical medicine and industrial production of white and
[0005] In the prior art, there is no report about the liquid symbiotic culture of mycorrhizal fungi and orchid seeds

Method used

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  • Method for rapid production of white and seedlings
  • Method for rapid production of white and seedlings
  • Method for rapid production of white and seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Identification of strains

[0043] 1. Extraction of fungal DNA:

[0044] The fungus used for molecular identification was inoculated on PDA medium and cultured in a dark room at 25°C for 2 weeks. When the diameter of the fungal colony was 3-5 cm, DNA could be extracted from the hyphae on the surface of the colony.

[0045] DNA extraction was performed according to the instructions of the Fungal DNA Extraction Kit (E.Z.N.ATM Fungal DNA Kit, Omega Bio-Tek, Doraville, Georgia, USA), and the specific steps were as follows:

[0046] 1) Scrape 50-100 mg mycelium into a sterilized mortar, add liquid nitrogen to freeze, grind it into powder immediately, and scrape the ground mycelium into a sterilized 1.5 mL Eppendorf tube.

[0047] 2) Add 800 μL of Buffer FG1 buffer solution to the Eppendorf tube, vortex to mix, and then put in a water bath at 65°C for 10 minutes, and invert and mix twice during the water bath.

[0048] 3) Then add 140 μL Buffer FG2, vortex and mix well, and...

Embodiment 2

[0084] The method for rapid production of Radix Radix Radix Radix Radixe of the present invention, comprises the following steps:

[0085] Step 1. Preparation of bacterial liquid: inoculate the mycorrhizal fungi into PDA solid medium, control the culture temperature at 28°C, and culture in dark for 10 days, then pick small pieces from the edge of the formed colony and inoculate them into the symbiotic liquid medium Shaker dark culture was carried out in the medium for 5 days, the speed of the shaker was 150r / min, and the culture temperature was 28°C to obtain the bacterial liquid of the mycelium of the mycorrhizal fungus;

[0086] Wherein, the symbiotic liquid medium is oat medium, wheat bran medium, potato medium, apple medium, radish medium, sweet potato medium, or taro medium;

[0087] Wherein the preparation method of oat culture medium is: weigh oat by 20g / L consumption, add the water of 500mL, keep 30min after boiling, after 4 layers of gauze filter, set the volume to 1L...

Embodiment 3

[0102] The method for rapid production of Radix Radix Radix Radix Radixe of the present invention, comprises the following steps:

[0103] Step 1. Preparation of bacterial liquid: inoculate the mycorrhizal fungus into PDA solid medium, control the culture temperature at 26°C, and culture in dark for 7 days, then pick small pieces from the edge of the formed colony and inoculate into the symbiotic liquid medium Dark culture was carried out on a shaking table in the medium for 3 days, the rotating speed of the shaking table was 100r / min, and the cultivation temperature was 26°C to obtain the bacterial liquid of the mycelium of the mycorrhizal fungi;

[0104] Wherein, the symbiotic liquid medium is oat medium, wheat bran medium, potato medium, apple medium, radish medium, sweet potato medium, or taro medium;

[0105] Wherein the preparation method of oat culture medium is: weigh oat by the consumption of 2g / L, add the water of 500mL, keep 30min after boiling, after 4 layers of ga...

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Abstract

The invention discloses a method for rapidly producing Bletilla striata germchit. The method comprises the steps of carrying out liquid co-culture on Bletilla striata seeds and Bletilla striata mycorrhiza fungi until the Bletilla striata seeds are germinated, and carrying out Bletilla striata germchit culture on the germinated Bletilla striata seeds, wherein the Bletilla striata mycorrhiza fungi are Sebacinales sp. mycorrhiza fungus strains YY-51 and are collected by General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on July 31, 2015, and have the collection number of GCMCC NO. 11104. The artificial Bletilla striata seeds or germchit produced by the method are easy in preservation and transportation, and the disadvantages of the traditional tissue culture process that the operation process is complicated, the technical requirements are high, repeated subculture is carried out, a transplanting or fix planting process needs a large number of manpower and materials, and the production cost is low are avoided.

Description

technical field [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a method for rapid production of Radix chinensis seedlings. Background technique [0002] Orchidaceae seeds are as small as dust, also known as "dust seeds". There are a large number of seeds, and each mature fruit contains more than tens of thousands of available seeds, but under natural conditions, the seeds are difficult to germinate. Orchidaceae are a special ecological group with a high degree of evolution in the plant kingdom and are closely related to fungi. Studies have shown that mycorrhizal fungi are inseparable from seed germination and plant growth of orchids; mycorrhizal fungi are particularly important in the protection, introduction, domestication and artificial cultivation of orchid medicinal and ornamental plants. Since most orchids are endangered, all wild species of orchids have been listed in the Convention on Internati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/10A01C1/02
CPCA01C1/02A01G18/10
Inventor 谭小明缪剑华李力周雅琴
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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