Method for rapid production of white and seedlings
A kind of seedling and rapid technology, applied in the biological field, can solve the problems of limited white and clinical medication, susceptibility to pathogenic bacteria, slow growth of sterile test tube seedlings, etc., achieve broad application prospects and economic value, avoid complicated operation process, and be easy to store and transport effects
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Embodiment 1
[0042] Identification of strains
[0043] 1. Extraction of fungal DNA:
[0044] The fungus used for molecular identification was inoculated on PDA medium and cultured in a dark room at 25°C for 2 weeks. When the diameter of the fungal colony was 3-5 cm, DNA could be extracted from the hyphae on the surface of the colony.
[0045] DNA extraction was performed according to the instructions of the Fungal DNA Extraction Kit (E.Z.N.ATM Fungal DNA Kit, Omega Bio-Tek, Doraville, Georgia, USA), and the specific steps were as follows:
[0046] 1) Scrape 50-100 mg mycelium into a sterilized mortar, add liquid nitrogen to freeze, grind it into powder immediately, and scrape the ground mycelium into a sterilized 1.5 mL Eppendorf tube.
[0047] 2) Add 800 μL of Buffer FG1 buffer solution to the Eppendorf tube, vortex to mix, and then put in a water bath at 65°C for 10 minutes, and invert and mix twice during the water bath.
[0048] 3) Then add 140 μL Buffer FG2, vortex and mix well, and...
Embodiment 2
[0084] The method for rapid production of Radix Radix Radix Radix Radixe of the present invention, comprises the following steps:
[0085] Step 1. Preparation of bacterial liquid: inoculate the mycorrhizal fungi into PDA solid medium, control the culture temperature at 28°C, and culture in dark for 10 days, then pick small pieces from the edge of the formed colony and inoculate them into the symbiotic liquid medium Shaker dark culture was carried out in the medium for 5 days, the speed of the shaker was 150r / min, and the culture temperature was 28°C to obtain the bacterial liquid of the mycelium of the mycorrhizal fungus;
[0086] Wherein, the symbiotic liquid medium is oat medium, wheat bran medium, potato medium, apple medium, radish medium, sweet potato medium, or taro medium;
[0087] Wherein the preparation method of oat culture medium is: weigh oat by 20g / L consumption, add the water of 500mL, keep 30min after boiling, after 4 layers of gauze filter, set the volume to 1L...
Embodiment 3
[0102] The method for rapid production of Radix Radix Radix Radix Radixe of the present invention, comprises the following steps:
[0103] Step 1. Preparation of bacterial liquid: inoculate the mycorrhizal fungus into PDA solid medium, control the culture temperature at 26°C, and culture in dark for 7 days, then pick small pieces from the edge of the formed colony and inoculate into the symbiotic liquid medium Dark culture was carried out on a shaking table in the medium for 3 days, the rotating speed of the shaking table was 100r / min, and the cultivation temperature was 26°C to obtain the bacterial liquid of the mycelium of the mycorrhizal fungi;
[0104] Wherein, the symbiotic liquid medium is oat medium, wheat bran medium, potato medium, apple medium, radish medium, sweet potato medium, or taro medium;
[0105] Wherein the preparation method of oat culture medium is: weigh oat by the consumption of 2g / L, add the water of 500mL, keep 30min after boiling, after 4 layers of ga...
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