A method for simultaneous quantitative detection of tea resin soluble pigment monomers
A quantitative detection and tea resin technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problem of unstable quantitative results, achieve the effect of enriching pigment types and improving accuracy
Active Publication Date: 2018-10-12
TEA RES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology
[0006] For the above-mentioned problems existing in the background technology, the purpose of the present invention is to provide a method for synchronous quantitative detection of tea resin-soluble pigment monomers, that is, to provide a simple technology for the separation and preparation of tea resin-soluble pigment components to solve the existing problems. The drawbacks of the technology overcome the shortcomings of the existing technology such as unstable quantitative results
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[0021] Monomer preparation: cut tea tree leaves into pieces, put them into a 2 mL centrifuge tube, add acetone and two 5 mm steel balls, use a ball mill at 30 Hz to grind for 1 min, and extract overnight at low temperature (4°C refrigerator) in the dark; centrifuge: Centrifuge at 12,000 r / min for 5 min; take 1 mL of the supernatant and put it into 2 ml of conical-bottom maximum recovery sample, and use a vacuum centrifugal concentrator to concentrate to about 50 uL; spot the concentrated solution on a G254 silica gel plate in a strip shape, petroleum Ether and acetone were mixed at a volume ratio of 3:2 as a developer for chromatography; the chromatography results are shown in figure 1 , the following monomers were obtained: 1: β-carotene; 2: γ-carotene; 3: pheophytin a; 4: pheophytin b; 5: chlorophyll a; 6: lutein; 7: chlorophyll b; 8: Violaxanthin; 9: Zeaxanthin; 10: Neoxanthin; 11: Pheophytin a; 12: Pheophytin b. Separate each color strip carefully and scrape it into a 2 m...
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The invention relates to a method for synchronously and quantitatively detecting tea resin soluble pigment monomer. The method is characterized by mainly comprising the following steps of (1) tea resin soluble pigment monomer separation; (2) pigment monomer qualitative and quantitative analysis; (3) detection. The method has the advantages that the effect of obtaining the soluble pigment monomer through preparation at any time is achieved; effective reference is provided for qualitative and quantitative analysis of tea resin soluble pigment; effective pigment monomer substances are provided for analysis by a liquid chromatography method; the analysis accuracy of the liquid chromatography method is improved; the variety of pigment detected by the liquid chromatography method is richened; the problem of mutual interference caused in unseparated mixed solution spectrum determination by a traditional spectrum method is solved.
Description
technical field [0001] The invention relates to a method for synchronously and quantitatively detecting tea resin-soluble pigment monomers. The method can be used in technical fields such as separation and extraction of tea pigment monomers, quantitative detection of tea resin-soluble pigment components, and the like. Background technique [0002] The analysis of fat-soluble pigments in tea is of great significance, but it is difficult to preserve because they are easily decomposed by light and easily oxidized and deteriorated. And fat-soluble pigment monomers play an important role in the qualitative and quantitative reference of pigment analysis. [0003] At present, the analysis of fat-soluble pigments mainly adopts colorimetric method and liquid chromatography. Colorimetry is usually performed directly on the pigment extract. This method does not use pigment monomer as a reference, and all pigment components are not pre-separated, and each pigment component interacts ...
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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 张群峰石元值马立锋伊晓云刘美雅倪康阮建云
Owner TEA RES INST CHINESE ACAD OF AGRI SCI