Anti-human amyloid beta-peptide nano antibody and application thereof

A nanobody and amyloid technology, applied in the fields of biochemistry and molecular biology, can solve the problems of increasing the cost of antibody preparation
CN105542005AInactive Publication Date: 2016-05-04DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN UNIV OF TECH
Publication Date
2016-05-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an anti-human amyloid beta-peptide nano antibody (Abeta) and application thereof. A bacteriophage display technique is utilized to carry out multi-screening on an alpaca bacteriophage antibody library to enrich the Abeta-specific bacteriophage; the bacteriophage is cultured to prepare the antibody; identification is carried out to obtain a positive clone; sequencing is carried out to obtain a corresponding coding sequence; and expression is carried out in Escherichia coli to obtain a soluble antibody segment. The antibody disclosed by the invention has the amino acid sequence disclosed as SEQ ID NO.8. The antibody disclosed by the invention has the specific combining capacity with Abeta, and can be used for preparing Abeta-targeted drugs for reagents for disease therapy and diagnosis.
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Description

technical field

[0001] The invention belongs to the field of biochemistry and molecular biology, and relates to a nanobody against human amyloid β peptide (amyloid β, Aβ) and application thereof. Background technique

[0002] Alzheimer's disease (AD) is a degenerative disease of the central nervous system characterized by progressive cognitive dysfunction and behavioral impairment. In September 2015, the Alzheimer'sdisease international, (ADI) report data showed that: at present, about one person suffers from Alzheimer's disease every 3.2 seconds, and 60-70% of the patients with Alzheimer's disease are Alzheimer's disease dementia. With the aging population As the base and proportion continue to increase, AD will bring more and more serious personal pain to patients and social and family pressure.

[0003] Amyloid β peptide (amyloidβ, Aβ) is a natural product present in the human brain, which is involved in the development of nerve cells (HeoC, et al. J Neurochem. 2007; 102...

Claims

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