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Solid tumor tissue digestive juice

A technology for tumor tissue and digestive juice, applied in the field of cell separation, can solve the problems that the digestion effect of tumor tissue needs to be improved, and achieve the effect of stable effect, good dispersion and convenient operation

Inactive Publication Date: 2016-05-04
THE FIRST AFFILIATED HOSPITAL OF XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The digestive effects of the various digestive juices in the prior art on tumor tissue still need to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A solid tumor tissue digestion solution prepared from the following components by weight:

[0019] 25 parts of type I collagenase, 45 parts of type I protein kinase, 1 part of dispase, 15 parts of hyaluronidase, 10 parts of poloxamer, 202.56 parts of buffer, superparamagnetic nanoparticle solution with a concentration of 15 mg / mL 10 servings. The buffer solution contains 5mmol / L tris-hydrochloric acid, 0.4mmol / L diaminoethane tetraacetic acid, 5mmol / L sodium chloride, 0.015μg·μl -1 Ethyl methanesulfonate.

Embodiment 2

[0021] A solid tumor tissue digestion solution prepared from the following components by weight:

[0022] 35 parts of type I collagenase, 55 parts of type I protein kinase, 4 parts of dispase, 25 parts of hyaluronidase, 30 parts of poloxamer, 320.35 parts of buffer, superparamagnetic nanoparticle solution with a concentration of 85mg / mL 20 servings. The buffer solution contains 15mmol / L tris-hydrochloric acid, 0.6mmol / L diaminoethane tetraacetic acid, 15mmol / L sodium chloride, 0.055μg·μl -1 Ethyl methanesulfonate.

Embodiment 3

[0024] A solid tumor tissue digestion solution prepared from the following components by weight:

[0025] 30 parts of type I collagenase, 50 parts of type I protein kinase, 2.5 parts of dispase, 20 parts of hyaluronidase, 20 parts of poloxamer, 261.455 parts of buffer solution, superparamagnetic nanoparticle solution with a concentration of 50 mg / mL 15 servings. The buffer solution contains 10mmol / L tris-hydrochloric acid, 0.5mmol / L diaminoethane tetraacetic acid, 10mmol / L sodium chloride, 0.035μg·μl -1 Ethyl methanesulfonate

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PUM

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Abstract

The invention discloses a solid tumor tissue digestive juice which is prepared from the following components in parts by weight: 25-35 parts of I type collagenase, 45-55 parts of I type protein kinase, 1-4 parts of dispase, 15-25 parts of hyaluronidase, 10-30 parts of poloxamer, 202.56-320.35 parts of buffer solution and 10-20 parts of 15-85 mg / mL superparamagnetic nanoparticle solution. The solid tumor tissue digestive juice has the advantages of small cytomembrane injury, favorable cell suspension dispersity and stable effects, and is convenient to operate.

Description

technical field [0001] The invention relates to the field of cell separation, in particular to a solid tumor tissue digestion liquid. Background technique [0002] When analyzing and studying the cell biological characteristics of solid tumor tissue (such as cell radiosensitivity detection, etc.), it is necessary to first prepare the sample tissue block into a dispersed single-cell suspension. Only when the various cell components in the sample tissue are in the state of single cells, can it be effectively detected and analyzed for various cell effects, and the quality of the cell suspension is closely related to the digestive solution formula and digestion method. The digestive effects of the various digestive juices in the prior art on tumor tissue still need to be improved. Contents of the invention [0003] In order to solve the above problems, the present invention provides a solid tumor tissue digestion solution, which can effectively and rapidly disperse solid tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/05C12N2500/30C12N2509/00
Inventor 杨留中寇卫政李秋萍杨庆辉蔡卫梅
Owner THE FIRST AFFILIATED HOSPITAL OF XINXIANG MEDICAL UNIV