Preparation method of electrochemical glucose biosensor and detection method for testing glucose

A biosensor, glucose technology, applied in the field of preparation of electrochemical biosensors, can solve problems such as lack, and achieve the effects of improving accuracy, high sensitivity, and widening the linear range

Active Publication Date: 2016-05-04
HENAN INST OF SCI & TECH
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is a lack of new preparation methods for electrochemical glucose sensors with high sensitivity and good stabi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of electrochemical glucose biosensor and detection method for testing glucose
  • Preparation method of electrochemical glucose biosensor and detection method for testing glucose
  • Preparation method of electrochemical glucose biosensor and detection method for testing glucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the electrochemical glucose biosensor of the present invention, through the following steps:

[0034] 1) Calcium titanate nanoparticles (CaTiO 3 ) dispersed in chitosan (CS) solution to prepare glucose oxidase (GOD) / calcium titanate nanoparticles / chitosan mixed solution (ie: GOD / CaTiO 3 / CS mixed solution);

[0035] 2) The GOD / CaTiO prepared in step 1) 3 / CS mixed solution was modified onto the surface of the pretreated glassy carbon electrode, and the electrochemical glucose biosensor was finally prepared.

Embodiment 2

[0037] The preparation method of the electrochemical glucose biosensor described in this example differs from Example 1 in that it further discloses the preparation process of calcium titanate nanoparticles in step 1):

[0038] a) Weigh a certain amount of calcium nitrate tetrahydrate (Ca(NO 3 ) 2 4H 2 O) and equal amounts of P25 titanium dioxide (P25TiO 2 ) placed in the crucible;

[0039] b) Heating the crucible to 550-650°C in a muffle furnace, maintaining the temperature for 9-11 hours, then cooling to room temperature, and collecting calcium titanate nanoparticles for use.

Embodiment 3

[0041] The preparation method of the electrochemical glucose biosensor described in this example differs from Example 1 or Example 2 in that in step 2), the pretreatment method for the glassy carbon electrode is as follows: the glassy carbon electrode is sequentially Polish with alumina powder with a particle size of 0.3 μm and 0.5 μm, then rinse off the residual alumina powder with deionized water, put it into dilute nitric acid aqueous solution for ultrasonic cleaning, and finally clean the glassy carbon electrode with ethanol and double distilled water in sequence, Blow dry quickly with nitrogen before retouching.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparation method of an electrochemical glucose biosensor and a detection method for testing glucose. The preparation method includes the steps of dispersing calcium titanate nanometer particles in a chitosan solution to prepare a GOD/CaTiO3/CS mixed solution; modifying the surface of a preprocessed glassy carbon electrode with the prepared GOD/CaTiO3/CS mixed solution to obtain the electrochemical glucose biosensor. The method for detecting a glucose sample through the electrochemical glucose biosensor prepared through the preparation method includes the following steps of firstly, adding the glucose sample to test liquid with the prepared modified electrode as the working electrode, a saturated calomel electrode as the reference electrode and a platinum sheet electrode as the counter electrode; secondly, detecting electrochemical signals of the working electrode through an electrochemical working station. Glucose oxidase is fixed through calcium titanate nanometer particles, and the biology catalytic activity of biological macromolecules can be well kept.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of an electrochemical glucose biosensor modified by calcium titanate nanoparticles, and a detection method using the electrochemical glucose biosensor. Background technique [0002] Diabetes is a serious threat to human health, so the diagnosis of diabetes is particularly important. By detecting the concentration of glucose in human blood, people's health status can be indirectly known, which provides a platform for the diagnosis of diabetes. [0003] At present, the methods for detecting glucose concentration include chromatography, spectrophotometry, polarimetry, colorimetry, etc. These analytical methods are low in sensitivity, laborious, and time-consuming, while the electrochemical biosensor method has higher sensitivity, and analysis It has the characteristics of fast speed, high selectivity, simple instrument operation and low pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 李永芳杨中智李长恭薛峰祝勇
Owner HENAN INST OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap