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Method for simultaneous determination of naringenin and eriodictyol content in fresh-cut water chestnut etiolation organization

A technology of naringenin and eriodictyol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of simultaneous determination of naringenin and eriodictol, etc., and achieve the effect of eliminating interference

Inactive Publication Date: 2016-05-11
HAINAN UNIVERSITY
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Problems solved by technology

[0003] In recent years, there have been separate literature reports on the detection and determination of naringenin and eriodictyol at home and abroad, mainly by high performance liquid chromatography (HPLC), or in combination with other methods, but the HPLC method mainly focuses on Chinese herbal medicines. The detection and content determination of naringenin and eriodictol in fresh-cut water chestnuts, and these conditions are not necessarily applicable to the detection and content determination of naringenin and eriodictol in the yellowed tissues of fresh-cut water chestnuts
In addition, there is no report on the determination of naringenin or eriodictyol in the yellowed tissues of fresh-cut water chestnuts by HPLC, let alone the simultaneous determination of naringenin and eriodictyol in the yellowed tissues of fresh-cut water chestnuts

Method used

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  • Method for simultaneous determination of naringenin and eriodictyol content in fresh-cut water chestnut etiolation organization
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  • Method for simultaneous determination of naringenin and eriodictyol content in fresh-cut water chestnut etiolation organization

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Embodiment

[0031] Step 1. Precisely weigh the standard naringenin 0.70±0.01mg and the standard saccharol 0.40±0.01mg into a 5mL brown volumetric flask, dissolve and dilute with methanol to the mark, shake well, and prepare the standard pummelo peel Stock solution and standard saccharol stock solution;

[0032] The precise standard naringenin stock solution and the standard saccharol stock solution 1mL each are placed in a 5mL brown volumetric flask, dissolved in methanol and diluted to the mark, shaken, and the concentration is naringenin 28±0.4μg·mL -1 , Saccharol 16±0.4μg·mL -1 The mixed standard solution should be stored at 4℃ and protected from light for later use.

[0033] Step 2. Take the standard mixed solution of naringenin and saccharol prepared in step 1 to prepare naringenin (5.60μg·mL -1 , 11.20μg·mL -1 , 16.80μg·mL -1 , 22.40μg·mL -1 , 28.00μg·mL -1 ), saccharol (3.20μg·mL -1 , 6.40μg·mL -1 , 9.60μg·mL -1 , 12.80μg·mL -1 , 16.00μg·mL -1 ) Determine the chromatographic peak area of...

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Abstract

The present invention discloses a method for simultaneous determination of naringenin and eriodictyol content in fresh-cut water chestnut etiolation organization, and the method comprises the six steps of preparation of a standard naringenin stock solution, preparation of a standard eriodictyol stock solution, preparation of a mixed naringenin and eriodictyol standard solution, production of a standard curve, preparation of a to-be-tested sample solution, and calculation of naringenin and eriodictyol content in a fresh-cut water chestnut etiolation organization sample. According to the method, the naringenin and the eriodictyol in the fresh-cut water chestnut etiolation organization can be effectively separated, the naringenin and eriodictyol content in the fresh-cut water chestnut etiolation organization can be simultaneously determined, the method is simple, fast, accurate and reliable, and can exclude interference produced by other substance chromatography and other substances contained in the fresh-cut water chestnut etiolation organization.

Description

Technical field [0001] The invention belongs to the technical field of element content determination, and specifically relates to a method for simultaneously determining the content of naringenin and saccharol in the yellowing tissue of fresh-cut water chestnuts. Background technique [0002] Eleocharistuberosa (Eleocharistuberosa), also known as horseshoe, water chestnut, ground chestnut, black taro, etc., is an underground bulb formed by perennial herbaceous water chestnut. Water chestnuts are not only crispy, juicy, and nutritious, but also can be used as medicine, which can assist in the treatment of many diseases. It has been known as "Underground Sydney" since ancient times, and northerners regard it as "Jiangnan Ginseng". However, after the water chestnuts are freshly cut, the cut surface will turn yellow in a short time, which seriously affects the appearance quality and other values. Earlier studies found that the main cause of yellowing was that certain substances in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34
CPCG01N30/02G01N30/34G01N2030/027
Inventor 潘永贵何凤平
Owner HAINAN UNIVERSITY
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