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Measuring method of TNF-alpha and IL-1alpha in blood by Fugongsan

A method of determination, Fugong powder technology, applied in the direction of medical formula, disease resistance to vector transmission, medical preparations containing active ingredients, etc., can solve the problems of no data explanation, etc., to achieve the goal of treating endometritis and reducing the impact Effect

Inactive Publication Date: 2016-05-18
CHONGQING THREE GORGES VOCATIONAL COLLEGE
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Problems solved by technology

At present, the treatment of this disease mainly relies on antibiotics, but with the continuous improvement of milk quality standards, the characteristics of traditional Chinese medicines such as mildness and low residue are becoming more and more prominent. Taohong Siwu Decoction is a classic pure Chinese medicine compound preparation for the treatment of endometritis. Powder is a traditional Chinese medicine preparation, which is prepared by adding several new herbs on the basis of classic prescriptions. The clinical application effect is good, but there is no data to explain

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  • Measuring method of TNF-alpha and IL-1alpha in blood by Fugongsan
  • Measuring method of TNF-alpha and IL-1alpha in blood by Fugongsan
  • Measuring method of TNF-alpha and IL-1alpha in blood by Fugongsan

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preparation example Construction

[0041] The present invention is a preparation method of Fugong Powder. The preparation method of Fugong Powder includes the following steps: preparing by water decocting and alcohol precipitation method, placing the medicinal materials in a beaker in proportion, adding 5 times the amount of the medicinal materials and soaking in distilled water for 1 hour, then Place it on an electric stove and boil for 1 hour, filter out the decoction, add appropriate amount of water to the dregs, decoct for 1.5 hours, combine the two decoctions and concentrate to 250ml, to make 1:1 (1g liquid is equivalent to 1g crude drug ), the crude drug content is 1g / mL, after autoclaving, it should be refrigerated for later use.

[0042] In the prepared 1:1 concentrated drug solution, 1 g of concentrated drug solution is equivalent to 1 g of crude drug.

[0043] The Fugong Powder of the present invention can significantly reduce the content of TNF-α and IL-1α in the blood of endometritis model rabbits (P<0.0...

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Abstract

The invention discloses a measuring method of TNF-alpha and IL-1alpha in blood by Fugongsan. The measuring method comprises the following steps: selecting pathogenic strains, grouping and molding; preparing Fugongsan and performing clinical observation, white blood cell differential counting, uterine secretion examination and content measurement of TNF-alpha and IL-1alpha; performing data analysis by using Excel and statistical software SPSS16.0 while marking with (average value)+ / -(standard error); and obtaining results and analyzing. In the invention, the Fugongsan can remarkably reduce the content (P<0.01) of TNF-alpha and IL-1alpha in the rabbit blood in an endometritis model while the reduction degree is positively correlated with the drug dose; and the Fugongsan is not obviously different from common traditional Chinese medicine anti-inflammatory agent Taohongsiwu decoction, indicating that the Fugongsan can reduce the influence of various inflammatory factors on the uterus and whole body and shows an anti-inflammatory effect so as to realize an effect of treating endometritis.

Description

Technical field [0001] The invention belongs to the technical field of medical medicaments, and particularly relates to a method for measuring TNF-α and IL-1α in blood by Fugong Powder. Background technique [0002] Endometritis is a common reproductive disease, which is one of the main reasons for the decline in reproductive performance and milk production of dairy cows. The main pathogens are pathogenic Escherichia coli and Staphylococcus aureus. At present, the treatment of this disease mainly relies on antibiotics, but with the continuous improvement of milk quality standards, the characteristics of traditional Chinese medicines such as mildness and low residues have become increasingly prominent. Taohong Siwu Decoction is a classic pure traditional Chinese medicine compound preparation for the treatment of endometritis. Powder is a traditional Chinese medicine preparation, which is prepared by adding several new medicines on the basis of classic prescriptions. The clinical a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9068A61K9/14A61P15/00A61K33/06
CPCA61K9/14A61K33/06A61K36/21A61K36/232A61K36/236A61K36/286A61K36/484A61K36/533A61K36/65A61K36/71A61K36/736A61K36/804A61K36/9068A61K2236/331A61K2236/51A61K2236/53A61K2236/55A61K2300/00Y02A50/30
Inventor 张传师彭津津雍康母治平杨庆稳王敬程文超周亚刘震坤周乾兰
Owner CHONGQING THREE GORGES VOCATIONAL COLLEGE
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