Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker

A chromosome arm and molecular marker technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of rapid variation of physiological races.

Active Publication Date: 2016-05-18
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physiological races of powdery mildew mutate quickly, and many disease-resistant genes are overcome by new races soon after they are used in production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker
  • Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker
  • Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1. Detection of whether wheat contains tufted wheat chromosome arms

[0086] 1. Preparation of a complete set of reagents for detecting whether wheat contains tufted wheat chromosome arms

[0087] The complete set of reagents for detecting whether wheat contains the chromosome arm of tufted wheat is set of reagent 1 and set of reagent 2, and the set of reagent 1 is composed of A-P, B-P, C-P, D-P and E-P;

[0088] A-P consists of two single-stranded DNAs shown in Sequence 1 and Sequence 2 in the sequence listing;

[0089] B-P consists of two single-stranded DNAs shown in Sequence 3 and Sequence 4 in the Sequence Listing;

[0090] C-P consists of two single-stranded DNAs shown in Sequence 5 and Sequence 6 in the sequence listing;

[0091] D-P consists of two single-stranded DNAs shown in sequence 7 and sequence 8 in the sequence listing;

[0092] E-P consists of two single-stranded DNAs shown in sequence 9 and sequence 10 in the sequence listing;

[0093] Kit ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and a molecular marker. The set reagent is composed of A-P and at lease one of B-P, C-P, D-P and E-P; the A-P is composed of two single-stranded DNA shown in a sequence 1 and a sequence 2 in a sequence list; the B-P is composed of two single-stranded DNA shown in a sequence 3 and a sequence 4 in the sequence list; the C-P is composed of two single-stranded DNA shown in a sequence 5 and a sequence 6 in the sequence list; the D-P is composed of two single-stranded DNA shown in a sequence 7 and a sequence 8 in the sequence list; the E-P is composed of two single-stranded DNA shown in a sequence 9 and a sequence 10 in the sequence list. The set reagent and a method can be used for screening a wheat cross population and identifying and assistantly breeding a new wheat line or variety with the excellent property (such as the powdery mildew resistance) of haynaldia villosa.

Description

technical field [0001] The invention relates to a complete set of reagents and molecular markers for detecting whether the genetic background of wheat contains the 6VS chromosome arm of tufted wheat in the field of biotechnology. Background technique [0002] Dasypyrumvillosum (L.) P. Candargy (syn. Haynaldiavillosa Schur) is a diploid species of the Triticum genus Dasypyrumvillosum, 2n=2x=14. It originated in the northeastern Mediterranean, from southern France in southern Europe to the Caspian Sea, southwestern Asia, Russia and the Caucasus (DePace et al. 1995; Frederiksen 1991), and is a weedy plant that grows in harsh, arid environments (AgnieszkaGradzielewska, 1991). 2006). It is named because of the clumps of hairs on the glume ridges and the top of the lemma. [0003] The tufted wheat contains many biotic and abiotic stress resistance genes and high-quality genes, and is an excellent gene source for improving wheat. Sears, Lukaszewski and Liu Dajun successively tra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 林志珊刘畅叶兴国王轲杜丽璞李仕金陈孝
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap