Microbial organic fertilizer for preventing and controlling strawberry red core disease, and production method thereof

A technology of microbial organic fertilizer and production method, which is applied in the treatment of biological organic parts, the preparation of organic fertilizers and organic fertilizers, etc. Significant effect, significant prevention and treatment effect

Inactive Publication Date: 2016-05-25
李德舜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, chemical control is mainly used for the prevention and treatment of strawberry red column disease, but the chemical control agent used for chemical control has a short duration and many application times. If the chemical control agent is used for a long time, the bacteria will easily develop resistance to it, and the control effect will be reduced. It will increase the pesticide residues in the fruit and affect food safety

Method used

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  • Microbial organic fertilizer for preventing and controlling strawberry red core disease, and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: be used for the preparation of the microbial organic fertilizer of preventing and treating strawberry red column disease:

[0029] 1. Materials:

[0030] 1.1 Bacterial strains: Streptomyces shandongensis is Streptomyces shandongensis recorded in the paper "Preliminary Study on Streptomyces hyperthermia 24#" (Li Deshun, Acta Microbiology 2004, 5th issue) of Shandong University; Strain with catalog number CICIMB1594.

[0031] 1.2 Medium:

[0032] LB medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH7.2, sterilized at 121°C for 20min.

[0033] Gaoshi No. 1 medium: by mass percentage, soluble starch 20‰, KNO 3 1‰, NaCl0.5‰, K 2 HPO 4 0.5‰, MgSO 4 0.5‰, FeSO 4 0.01‰, agar 20g, water 1000ml, pH7.2-7.4.

[0034] Fermentation medium: bran 10g / L, bean cake powder 6g / L, corn flour 10g / L.

[0035] 2. Preparation method:

[0036] (1) Mix chicken manure and mushroom dregs according to the mass ratio of 45:50, and carry out composting and fer...

Embodiment 2

[0043] Embodiment 2: the prevention and treatment effect of microbial organic fertilizer of the present invention to strawberry red column disease

[0044] Select the diseased field of strawberry red column disease, and design three treatments: treatment 1: control (contrast CK is the application of common organic fertilizer); treatment 2: application of compound Trichoderma (Guangzhou Microbiology Technology Co., Ltd.); treatment 3: application of The microbial organic fertilizer prepared in Example 1 of the present invention.

[0045] Each treatment was repeated 3 times, and the application rate was 50Kg / mu, and statistical analysis was carried out after 30 days of application.

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Abstract

The invention discloses a microbial organic fertilizer for preventing and controlling strawberry red core disease, and a production method thereof. The method comprises the following steps: respectively processing Streptomyces shandongensis sp.nov and Bacillus thermoamylovorans to prepare a primary seed liquid and a secondary seed liquid, carrying out fermentation culture, mixing the above fermentation cultured bacterial liquids according to a (1-3):(5-10), adding the obtained bacterial liquid mixture to organic matters obtained after primary compost fermentation of manure and mushroom residues, and re-fermenting the obtained material, wherein the addition amount of the bacterial liquid mixture is 10-20%; and carrying out low temperature drying on the re-fermented culture to obtain the microbial organic fertilizer. No antagonistic action exists between microbial strains selected by the microbial organic fertilizer, and all strains and metabolites thereof have synergism, can commonly inhibit propagation of plant pathogens to realize pest disease prevention and control effects, especially have substantial prevention and control effects on the strawberry red core disease, are fast effective, and generate no drug resistance.

Description

technical field [0001] The invention relates to a microbial organic fertilizer for preventing and treating strawberry red column disease and a production method thereof. Background technique [0002] Strawberry root rot in solar greenhouse (also known as red heart disease, red heart root rot, brown heart disease) is a root disease that has occurred on a large scale in greenhouse strawberry production in recent years. In Hebei, Liaoning, Shandong and other main strawberry planting areas, the damage is serious, and there are two common types: acute type and chronic type. The acute type usually occurs in spring and summer. After the rain, the tip of the leaves suddenly withers, and soon becomes green and withered, causing the whole plant to die quickly. The chronic type can occur after colonization to the beginning of winter. The margins of the lower old leaves turn purple red or purple brown, gradually expand upward, and the whole plant wilts or dies. The occurrence of straw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F17/00
CPCY02W30/40
Inventor 李德舜张海林李瑾
Owner 李德舜
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