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Lily X virus semi-quantitative detection gold-labeled card and preparation method thereof

A semi-quantitative detection, gold standard card technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve high accuracy, good repeatability, and strong specificity

Active Publication Date: 2016-05-25
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, the current prevention and control of lily virus is mainly based on killing the transmission medium - aphids. How to scientifically spray anti-lily virus agents, whether the proliferation of lily virus is inhibited after spraying anti-viral agents, and if it has been inhibited, what is the reason for the inhibition? There are no relevant reports on the extent of the problem, and the solution to these problems first depends on semi-quantitative detection; therefore, the realization of semi-quantitative detection is of great significance

Method used

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  • Lily X virus semi-quantitative detection gold-labeled card and preparation method thereof
  • Lily X virus semi-quantitative detection gold-labeled card and preparation method thereof

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Embodiment Construction

[0023] Such as figure 1 and figure 2 The LVX semi-quantitative detection gold standard card shown includes a gold standard card slot 1, a backing plate 12, a sample pad 8, a colloidal gold binding pad 9, a nitrocellulose membrane 10, and a water-absorbing filter paper 11, wherein the gold standard card slot 1 includes the upper The casing and the lower casing, the upper casing and the lower casing are connected by buckles, the upper casing is provided with a sample injection hole 2 and a reaction window 3, the sample pad 8 is placed under the sample injection hole 2, and the nitrocellulose membrane 10 is placed Below the reaction window 3, the gold standard probe is contained on the colloidal gold binding pad 9, the backing plate 12 is fixed in the gold standard card slot 1, the sample pad 8, the colloidal gold binding pad 9, the nitrocellulose membrane 10 and the water-absorbing filter paper 11 are sequentially Arranged and connected to the upper surface of the liner 12, t...

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PUM

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Abstract

A lily X virus semi-quantitative detection gold-labeled card and a preparation method thereof are disclosed, in order to improve use efficiency and in-field popularizing rate of colloidal gold immune chromatography test method for lily virus fast and semi-quantitative detection, the number of detection lines is increased to 3, then a known amount of antibodies with concentration differences are respectively coated in the detection lines, the lily X virus fast and semi-quantitative detection gold-labeled card can be developed, lily large-scale detoxification and lily X virus fast and in-field semi-quantitative detection needs can be met, the lily X virus fast and semi-quantitative detection gold-labeled card is strongly targeted, easy in operation, high in accuracy and strong in sensitivity, and virus content difference of samples can accurately be detected without using of any instruments and equipment.

Description

technical field [0001] The present invention relates to a gold standard card for rapid and semi-quantitative detection of lily virus and its preparation method, specifically referring to the development of a gold standard card capable of rapid and semi-quantitative detection of lily virus X (LVX) by colloidal gold immunochromatography and its preparation method . Background technique [0002] lily( Lilium spp. ) is the Liliaceae ( Liliaceae ) Lilium ( Lilium ) is a perennial monocotyledonous herbaceous plant. It is a world-renowned bulb flower with a long history of cultivation. It integrates ornamental, edible and medicinal uses. As the largest flower planting country in the world, the Netherlands has a relatively mature lily cut flower and bulb industry. ; As of 2005, the planting area of ​​cut flower lily in the Netherlands reached 3,800 hectares, accounting for about 72% of the global production of lily bulbs, and the output value reached 150 million euros; while in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/531G01N33/532G01N21/78
CPCG01N21/78G01N33/531G01N33/532G01N33/558G01N33/56983
Inventor 张玉宝王亚军谢忠奎王若愚王乐郭志鸿
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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