Direct seeding method of bletilla ochracea protocorm

A technique of protocorm and yellow flower is applied in the field of direct seeding propagation of the protocorm of the medicinal plant Huanghuabaiji to achieve the effects of improving the germination rate and reducing the production cost.

Active Publication Date: 2016-06-01
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the problems existing in the existing chrysanthemum and planting, and t

Method used

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  • Direct seeding method of bletilla ochracea protocorm
  • Direct seeding method of bletilla ochracea protocorm
  • Direct seeding method of bletilla ochracea protocorm

Examples

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Example Embodiment

[0023] Example 1

[0024] 1. Yellow flower white and seed germination

[0025] Select mature yellow flowers and fruit pods, sterilize them with sterilized water, mercury, and 75% ethanol aqueous solution, then peel off the fruit pods and take out the yellow flowers and seeds, and evenly spread the seeds on the yellow flowers in the tissue culture bottle On white and white culture medium, culture for 70 days in a tissue culture room with a temperature of 25±2℃, light intensity of 2500Lx, and 12h / 12h light to make the seeds green and swell and germinate into protocorms. Some roots and buds will be produced on the protocorms. , Then move the tissue culture bottle to the greenhouse seedbed for 7 days to refine the seedlings, gently take out the protocorm, and rinse the medium attached to it with water. Among them, Huanghuabai and the medium are mixed with 6-benzylamino adenine, naphthalene acetic acid, and banana juice in MS medium. The volume is made up with distilled water, and then...

Example Embodiment

[0034] Example 2

[0035] 1. Yellow flower white and seed germination

[0036] Select mature yellow flowers and fruit pods, sterilize them with sterilized water, mercury, and 75% ethanol aqueous solution, then peel off the fruit pods and take out the yellow flowers and seeds, and evenly spread the seeds on the yellow flowers in the tissue culture bottle On white and white culture medium, culture for 70 days in a tissue culture room with a temperature of 25±2℃, a light intensity of 2000Lx, and a light of 12h / 12h. The seeds will become green and swell and germinate into protocorms. Some roots and buds will be produced on the protocorms. , Then move the tissue culture bottle to the greenhouse seedbed for 7 days to refine the seedlings, gently take out the protocorm, and rinse the medium attached to it with water. Among them, Huanghuabai and the medium are mixed with 6-benzylamino adenine, naphthalene acetic acid, and banana juice in MS medium. The volume is made up with distilled wat...

Example Embodiment

[0045] Example 3

[0046] 1. Yellow flower white and seed germination

[0047] Select mature yellow flowers and fruit pods, sterilize them with sterilized water, mercury, and 75% ethanol aqueous solution, then peel off the fruit pods and take out the yellow flowers and seeds, and evenly spread the seeds on the yellow flowers in the tissue culture bottle On white and white culture medium, culture for 70 days in a tissue culture room with temperature of 25±2℃, light intensity of 3000Lx, light of 12h / 12h, so that the seeds will become green and swell and germinate into protocorms, and some of the protocorms will produce roots and buds. , Then move the tissue culture bottle to the greenhouse seedbed to refine the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, Huanghuabai and the medium are mixed with 6-benzylamino adenine, naphthalene acetic acid, and banana juice in MS medium. The volume is made up with distilled water...

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Abstract

The invention discloses a direct seeding method of bletilla ochracea protocorm. According to the method, firstly, bletilla ochracea seeds grow into protocorm under the tissue culture condition; then the protocorm cultured through tissue culture is directly seeded for growing seedlings on a seedbed in a greenhouse. The method is easy to implement, the seeds fast sprout first under the tissue culture condition, when the protocorm needs to be subjected to transplantation culture, the protocorm is transplanted in the nature, the germination rate is increased, production cost is reduced, lots of bletilla ochracea seedlings can be acquired in a short time, the requirement of large-area and large-scale seedling growing is met, and the method has dual significance in production and economy.

Description

technical field [0001] The invention belongs to the technical field of artificial planting of medicinal plants, and in particular relates to a protocorm direct seeding propagation method of the medicinal plant chrysanthemum chinensis. Background technique [0002] Bletillaochracea (Bletillaochracea) is a perennial herbaceous plant belonging to Orchidaceae, mainly distributed in the vast areas south of the Qinling Mountains in my country, and most of them are Shangluo, Zhashui and Hanzhong in Shaanxi. In recent years, due to its excellent efficacy in whitening and moisturizing, it has gradually attracted people's attention. In addition, it also has good development value in chemical industry. [0003] There are about 30,000 seeds in the chrysanthemum and its fruit pods, but the germination rate of the seeds is low or even not germinated under natural conditions. Therefore, the solution to the chrysanthemum and the shortage of resources is mainly through tissue culture. The t...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G24/00A01G31/00
Inventor 王喆之张慧牛俊峰肖娅萍王尚萍吴玲玲
Owner SHAANXI NORMAL UNIV
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