Direct seeding method of bletilla ochracea protocorm
A technique of protocorm and yellow flower is applied in the field of direct seeding propagation of the protocorm of the medicinal plant Huanghuabaiji to achieve the effects of improving the germination rate and reducing the production cost.
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Embodiment 1
[0024] 1. Yellow flower and seed germination
[0025] Select mature Chrysanthemum chrysalis and fruit pods, sterilize with sterilized water, mercury chloride, and ethanol solution with a volume fraction of 75% in sequence, then peel off the pods to take out Chrysanthemum chrysogenum and seeds, and spread the seeds evenly on the chrysanthemum in the tissue culture bottle Cultivate for 70 days in a tissue culture room with a temperature of 25±2°C, a light intensity of 2500Lx, and a light intensity of 12h / 12h on the white medium, so that the seeds turn green and swell and germinate into protocorms, and some protocorms will produce roots and buds , and then move the tissue culture bottle to the greenhouse seedbed to harden the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, the Huanghuabai culture medium is to add 6-benzylaminoadenine, naphthaleneacetic acid, and banana juice to the MS medium, use distilled water to ...
Embodiment 2
[0035] 1. Yellow flower and seed germination
[0036] Select mature Chrysanthemum chrysalis and fruit pods, sterilize with sterilized water, mercury chloride, and ethanol solution with a volume fraction of 75% in sequence, then peel off the pods to take out Chrysanthemum chrysogenum and seeds, and spread the seeds evenly on the chrysanthemum in the tissue culture bottle Cultivate for 70 days in a tissue culture room with a temperature of 25±2°C, a light intensity of 2000Lx, and a light intensity of 12h / 12h on the white medium, so that the seeds turn green and swell and germinate into protocorms, and some protocorms will produce roots and buds , and then move the tissue culture bottle to the greenhouse seedbed to harden the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, the Huanghuabai culture medium is to add 6-benzylaminoadenine, naphthaleneacetic acid, and banana juice to the MS medium, use distilled water to ...
Embodiment 3
[0046] 1. Yellow flower and seed germination
[0047] Select mature Chrysanthemum chrysalis and fruit pods, sterilize with sterilized water, mercury chloride, and ethanol solution with a volume fraction of 75% in sequence, then peel off the pods to take out Chrysanthemum chrysogenum and seeds, and spread the seeds evenly on the chrysanthemum in the tissue culture bottle Cultivate for 70 days in a tissue culture room with a temperature of 25±2°C, a light intensity of 3000Lx, and a light intensity of 12h / 12h on the white medium, so that the seeds turn green and swell and germinate into protocorms, and some protocorms will produce roots and buds , and then move the tissue culture bottle to the greenhouse seedbed to harden the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, the Huanghuabai culture medium is to add 6-benzylaminoadenine, naphthaleneacetic acid, and banana juice to the MS medium, use distilled water to ...
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