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Direct seeding method of bletilla ochracea protocorm

A technique of protocorm and yellow flower is applied in the field of direct seeding propagation of the protocorm of the medicinal plant Huanghuabaiji to achieve the effects of improving the germination rate and reducing the production cost.

Active Publication Date: 2016-06-01
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the problems existing in the existing chrysanthemum and planting, and to provide a method with low production cost, high germination rate and the ability to obtain a large amount of chrysanthemum and seedlings in a short time

Method used

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  • Direct seeding method of bletilla ochracea protocorm
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  • Direct seeding method of bletilla ochracea protocorm

Examples

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Effect test

Embodiment 1

[0024] 1. Yellow flower and seed germination

[0025] Select mature Chrysanthemum chrysalis and fruit pods, sterilize with sterilized water, mercury chloride, and ethanol solution with a volume fraction of 75% in sequence, then peel off the pods to take out Chrysanthemum chrysogenum and seeds, and spread the seeds evenly on the chrysanthemum in the tissue culture bottle Cultivate for 70 days in a tissue culture room with a temperature of 25±2°C, a light intensity of 2500Lx, and a light intensity of 12h / 12h on the white medium, so that the seeds turn green and swell and germinate into protocorms, and some protocorms will produce roots and buds , and then move the tissue culture bottle to the greenhouse seedbed to harden the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, the Huanghuabai culture medium is to add 6-benzylaminoadenine, naphthaleneacetic acid, and banana juice to the MS medium, use distilled water to ...

Embodiment 2

[0035] 1. Yellow flower and seed germination

[0036] Select mature Chrysanthemum chrysalis and fruit pods, sterilize with sterilized water, mercury chloride, and ethanol solution with a volume fraction of 75% in sequence, then peel off the pods to take out Chrysanthemum chrysogenum and seeds, and spread the seeds evenly on the chrysanthemum in the tissue culture bottle Cultivate for 70 days in a tissue culture room with a temperature of 25±2°C, a light intensity of 2000Lx, and a light intensity of 12h / 12h on the white medium, so that the seeds turn green and swell and germinate into protocorms, and some protocorms will produce roots and buds , and then move the tissue culture bottle to the greenhouse seedbed to harden the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, the Huanghuabai culture medium is to add 6-benzylaminoadenine, naphthaleneacetic acid, and banana juice to the MS medium, use distilled water to ...

Embodiment 3

[0046] 1. Yellow flower and seed germination

[0047] Select mature Chrysanthemum chrysalis and fruit pods, sterilize with sterilized water, mercury chloride, and ethanol solution with a volume fraction of 75% in sequence, then peel off the pods to take out Chrysanthemum chrysogenum and seeds, and spread the seeds evenly on the chrysanthemum in the tissue culture bottle Cultivate for 70 days in a tissue culture room with a temperature of 25±2°C, a light intensity of 3000Lx, and a light intensity of 12h / 12h on the white medium, so that the seeds turn green and swell and germinate into protocorms, and some protocorms will produce roots and buds , and then move the tissue culture bottle to the greenhouse seedbed to harden the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, the Huanghuabai culture medium is to add 6-benzylaminoadenine, naphthaleneacetic acid, and banana juice to the MS medium, use distilled water to ...

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Abstract

The invention discloses a direct seeding method of bletilla ochracea protocorm. According to the method, firstly, bletilla ochracea seeds grow into protocorm under the tissue culture condition; then the protocorm cultured through tissue culture is directly seeded for growing seedlings on a seedbed in a greenhouse. The method is easy to implement, the seeds fast sprout first under the tissue culture condition, when the protocorm needs to be subjected to transplantation culture, the protocorm is transplanted in the nature, the germination rate is increased, production cost is reduced, lots of bletilla ochracea seedlings can be acquired in a short time, the requirement of large-area and large-scale seedling growing is met, and the method has dual significance in production and economy.

Description

technical field [0001] The invention belongs to the technical field of artificial planting of medicinal plants, and in particular relates to a protocorm direct seeding propagation method of the medicinal plant chrysanthemum chinensis. Background technique [0002] Bletillaochracea (Bletillaochracea) is a perennial herbaceous plant belonging to Orchidaceae, mainly distributed in the vast areas south of the Qinling Mountains in my country, and most of them are Shangluo, Zhashui and Hanzhong in Shaanxi. In recent years, due to its excellent efficacy in whitening and moisturizing, it has gradually attracted people's attention. In addition, it also has good development value in chemical industry. [0003] There are about 30,000 seeds in the chrysanthemum and its fruit pods, but the germination rate of the seeds is low or even not germinated under natural conditions. Therefore, the solution to the chrysanthemum and the shortage of resources is mainly through tissue culture. The t...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G24/00A01G31/00
Inventor 王喆之张慧牛俊峰肖娅萍王尚萍吴玲玲
Owner SHAANXI NORMAL UNIV
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