Direct seeding method of bletilla ochracea protocorm
A technique of protocorm and yellow flower is applied in the field of direct seeding propagation of the protocorm of the medicinal plant Huanghuabaiji to achieve the effects of improving the germination rate and reducing the production cost.
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[0023] Example 1
[0024] 1. Yellow flower white and seed germination
[0025] Select mature yellow flowers and fruit pods, sterilize them with sterilized water, mercury, and 75% ethanol aqueous solution, then peel off the fruit pods and take out the yellow flowers and seeds, and evenly spread the seeds on the yellow flowers in the tissue culture bottle On white and white culture medium, culture for 70 days in a tissue culture room with a temperature of 25±2℃, light intensity of 2500Lx, and 12h / 12h light to make the seeds green and swell and germinate into protocorms. Some roots and buds will be produced on the protocorms. , Then move the tissue culture bottle to the greenhouse seedbed for 7 days to refine the seedlings, gently take out the protocorm, and rinse the medium attached to it with water. Among them, Huanghuabai and the medium are mixed with 6-benzylamino adenine, naphthalene acetic acid, and banana juice in MS medium. The volume is made up with distilled water, and then...
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[0034] Example 2
[0035] 1. Yellow flower white and seed germination
[0036] Select mature yellow flowers and fruit pods, sterilize them with sterilized water, mercury, and 75% ethanol aqueous solution, then peel off the fruit pods and take out the yellow flowers and seeds, and evenly spread the seeds on the yellow flowers in the tissue culture bottle On white and white culture medium, culture for 70 days in a tissue culture room with a temperature of 25±2℃, a light intensity of 2000Lx, and a light of 12h / 12h. The seeds will become green and swell and germinate into protocorms. Some roots and buds will be produced on the protocorms. , Then move the tissue culture bottle to the greenhouse seedbed for 7 days to refine the seedlings, gently take out the protocorm, and rinse the medium attached to it with water. Among them, Huanghuabai and the medium are mixed with 6-benzylamino adenine, naphthalene acetic acid, and banana juice in MS medium. The volume is made up with distilled wat...
Example Embodiment
[0045] Example 3
[0046] 1. Yellow flower white and seed germination
[0047] Select mature yellow flowers and fruit pods, sterilize them with sterilized water, mercury, and 75% ethanol aqueous solution, then peel off the fruit pods and take out the yellow flowers and seeds, and evenly spread the seeds on the yellow flowers in the tissue culture bottle On white and white culture medium, culture for 70 days in a tissue culture room with temperature of 25±2℃, light intensity of 3000Lx, light of 12h / 12h, so that the seeds will become green and swell and germinate into protocorms, and some of the protocorms will produce roots and buds. , Then move the tissue culture bottle to the greenhouse seedbed to refine the seedlings for 7 days, gently take out the protocorm, and rinse the medium attached to it with water. Among them, Huanghuabai and the medium are mixed with 6-benzylamino adenine, naphthalene acetic acid, and banana juice in MS medium. The volume is made up with distilled water...
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