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Alpha-galactosidase soft capsule feed additive and preparing method thereof

A feed additive, galactosidase technology, applied in the field of feed additives, can solve problems such as α-Gal decomposition

Active Publication Date: 2016-06-08
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that α-galactosidase has a catalytic blank period in the digestion process of fed animals, and α-Gal will be decomposed by gastric juice during this period, the invention provides an α-galactosidase soft capsule feed Additive and its preparation method

Method used

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  • Alpha-galactosidase soft capsule feed additive and preparing method thereof
  • Alpha-galactosidase soft capsule feed additive and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Add 10wt% α-Gal of the phosphate buffer solution to the phosphate buffer solution, then add β-CD until the concentration of β-CD reaches 10mmol / L, then leave it to react after homogenization, and the reaction time is 90min, the reaction temperature is 35°C, the pH value at the end of the reaction is 6.5, and the α-Gal—β-CD additive solution is obtained;

[0019] 2) Dissolve 100 parts of B-type gelatin in 90 parts of water, fully swell, add 30 parts of glycerin, 1.5 parts of preservative and 1.5 parts of titanium dioxide, mix evenly for gelation, the gelation temperature is 75 ° C, and then Evaporate 90% of the water at 85°C to obtain the rubber compound;

[0020] 3) Use the α-Gal-β-CD additive liquid obtained in step 1) as the soft capsule additive liquid, and use the rubber material obtained in step 2) as the soft capsule rubber material for filling to obtain α-galactosidase soft capsule feed additive.

Embodiment 2

[0022] 1) Add 12wt% α-Gal of the phosphate buffer solution to the phosphate buffer solution, then add β-CD until the concentration of β-CD reaches 10mmol / L, then leave it to react after homogenization, and the reaction time is 120min, the reaction temperature is 32°C, the pH value at the end of the reaction is 7, and the α-Gal—β-CD additive solution is obtained;

[0023] 2) Dissolve 100 parts of type B gelatin in 90 parts of water, fully swell, add 20 parts of glycerin, 1 part of preservative and 2 parts of titanium dioxide, mix evenly for gelation, the gelation temperature is 80 °C, and then Evaporate 94% of the water at 80°C to obtain the rubber compound;

[0024] 3) Use the α-Gal-β-CD additive liquid obtained in step 1) as the soft capsule additive liquid, and use the rubber material obtained in step 2) as the soft capsule rubber material for filling to obtain α-galactosidase soft capsule feed additive

Embodiment 3

[0026] 1) Add the α-Gal of described phosphate buffer solution 10wt% to the phosphate buffer solution, then add β-CD until the concentration of β-CD reaches 12mmol / L, then stand for reaction after homogenization, the reaction time is 60min, the reaction temperature is 38°C, the pH value at the end of the reaction is 6, and the α-Gal—β-CD additive solution is obtained;

[0027] 2) Dissolve 100 parts of B-type gelatin in 95 parts of water, fully swell, add 40 parts of glycerin, 2 parts of preservative and 1 part of titanium dioxide, mix evenly for gelation, the gelation temperature is 70 ° C, and then Evaporate 92% of the water at 90°C to obtain the rubber compound;

[0028] 3) Use the α-Gal-β-CD additive liquid obtained in step 1) as the soft capsule additive liquid, and use the rubber material obtained in step 2) as the soft capsule rubber material for filling to obtain α-galactosidase soft capsule feed additive.

[0029] Effect example

[0030] Taking the additives provide...

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Abstract

The invention belongs to the technical field of feed additives, and particularly relates to an alpha-galactosidase soft capsule feed additive and a preparing method thereof. The method includes the following steps that alpha-Gal and beta-CD are added into a phosphate buffered solution, and the mixed solution is homogenized and stands for a reaction to a reaction endpoint to obtain alpha-Gal-beta-CD additive solution; 2, B-type gelatin is added into water, and glycerinum, preservative and titanium dioxide are added after the B-type gelatin is swelled fully and are mixed uniformly to conduct colloidizing to obtain glue stock; 3, filling is conducted. The inhibition action of cyclodextrin inhibitor to alpha-Gal is used, so that alpha-Gal enzyme activity in animal feed is reduced and then improved, and alpha-Gal in the feed is more suitable for having the catalysis effect in the digestion period.

Description

technical field [0001] The invention belongs to the technical field of feed additives, in particular to an alpha-galactosidase soft capsule feed additive and a preparation method thereof. Background technique [0002] α-galactosidase (α-galactosidase, α-Gal) is an exoglycosidase that catalyzes the hydrolysis of α-galactosidic bonds. It is also known as melibiose because it can decompose melibiose. - Hydrolysis of galactosidic linkages. This feature makes it useful for improving and eliminating anti-nutritional components in feed and soy-based foods. [0003] Corn-soybean meal-based feed is a good source of protein feed due to its nutritional characteristics such as high crude protein content, balanced amino acid content and rich in lysine. However, the content of Ⅱ-galactoside sugar in soybean meal and miscellaneous meal feed is relatively high, and it is an anti-nutritional factor in feed. Its anti-nutritional effect is often ignored, and it is difficult to decompose it b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/189A23K40/30
Inventor 沈汪洋孙威李芳陈轩蔡红燕孙启发
Owner WUHAN POLYTECHNIC UNIVERSITY
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