Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A rapid propagation method induced by somatic embryos in isolated leaves of Sophora japonica

A technology of detached leaves and somatic embryos, applied in the field of plant biology, can solve the problems of less budding and low regeneration rate of Sophora japonica, and achieve the effect of saving time

Active Publication Date: 2018-05-25
SHANDONG FOREST SCI RES INST
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the above problems, and to provide a rapid propagation method for the induction of somatic embryos from isolated leaves of Sophora japonica, so as to solve the problems of low regeneration rate and few buds of Sophora japonica.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A rapid propagation method induced by somatic embryos in isolated leaves of Sophora japonica
  • A rapid propagation method induced by somatic embryos in isolated leaves of Sophora japonica
  • A rapid propagation method induced by somatic embryos in isolated leaves of Sophora japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Select a single plant with strong growth and excellent performance, and take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0049] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Three stem segments were inoculated in each bottle to induce the germination of terminal buds and axillary buds. After 15 days of cultivation, the axillary buds or terminal buds of the stems begin to ge...

Embodiment 2

[0061] Select a single plant with strong growth and excellent performance, and take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0062] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 40 seconds, rinse with sterile water for 4 times, then sterilize with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 6 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Inoculate 3 stem segments per bottle to induce bud germination. After 18 days of cultivation, the axillary buds or terminal buds of the stems begin to germinate, and the stem tips elongate obviously, an...

Embodiment 3

[0073] Select a single plant with strong growth and excellent performance, and take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0074] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 60 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercury chloride for 15 minutes, rinse with sterile water for 6 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Inoculate 3 stem segments per bottle to induce bud germination. After 20 days of cultivation, the axillary buds or terminal buds of the stems begin to germinate, and the stem tips elongate obviously, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Chinese scholartree detached leaf somatic embryo induction rapid-breeding method. The method comprises selecting Chinese scholartree tender twigs, disinfecting the tender twigs, taking stems with axillary buds or terminal buds, inoculating a bud initial medium with the axillary buds or terminal buds, inducing germination of the axillary buds or terminal buds, removing base leaves of the produced young sprouts, carrying out cutting to obtain stem sections with axillary buds or terminal buds, inoculating a test-tube plantlet multiplication medium with the stem sections with axillary buds or terminal buds to obtain sterile test-tube plantlets, taking leaves of the sterile test-tube plantlets, inoculating a somatic embryo inducing medium with the leaves of the sterile test-tube plantlets, carrying out culture to obtain callus, inoculating an adventitious bud induction medium with the callus, carrying out culture to obtain multiple shoots, cutting the multiple shoots to obtain callus blocks, inoculating a test-tube plantlet multiplication medium or an adventitious bud induction medium with the callus blocks, carrying out culture, transferring the cultured multiple shoots into a shoot strengthening medium, carrying out shoot strengthening culture and carrying out seedling hardening and transplanting. The method has a high regeneration rate, a large budding rate, a plant transplanting survival rate of 95% or more, produces strong seedlings and effectively solves the problem of degeneration of excellent seedling germplasm.

Description

technical field [0001] The invention relates to a rapid propagation method for induction of somatic embryos from isolated leaves of Sophora japonica, belonging to the field of plant biotechnology. Background technique [0002] Sophora japonica Linn, also known as Sophora japonica Linn, also known as Sophora japonica Linn, belongs to Papilionaceae and Sophora genus. Chinese pagoda tree is a deciduous tree with beautiful tree vigor, long flowering period, extremely resistant to pruning, cold resistance, drought resistance, and air pollution resistance. It is also a symbol of auspiciousness, happiness and beauty. It is an ideal tree species for street and garden greening. The material is excellent, the flowers, fruits, root bark, and bark can be used as medicine, and the seeds can be squeezed to make soap. It has high economic value and has a very broad application prospect. At present, the national pagoda tree is the only rootstock tree species for grafting ornamental species...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李双云杨国良李丽庞彩红刘盛芳王守国张炳孝夏阳
Owner SHANDONG FOREST SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com