Method for marking gastrointestinal tract of zebra fish with calcein
A calcein, gastrointestinal technology, applied in the field of drug screening, can solve problems such as long experimental period, achieve the effects of intuitive observation, enhanced peristalsis and feeding ability, and improved accuracy and reliability
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Embodiment 1
[0055] Example 1: Labeling the gastrointestinal tract of zebrafish with different concentrations of calcein
[0056] 1. Zebrafish sample pretreatment:
[0057] Use healthy and sexually mature AB zebrafish, put them into a mating tank at a ratio of 1:1 or 1:2, place a partition in the middle, and place them in a dark environment. Remove the partition before turning on the light the next day, and light stimulate Make it ovulate, fish out adult fish after ovulation half an hour, control the ovulation time within half an hour, to reduce the difference in development time between embryos. Collect the fertilized eggs and rinse them three times with new zebrafish embryo culture water to disinfect and clean the fertilized eggs; then transfer the fertilized eggs into clean water for zebrafish embryo culture, and control the temperature at 28°C with a 14h light / 10h dark cycle. Culture in light, change about 1 / 3 of the water every 24 hours, and suck out the dead embryos in time. The 6-...
Embodiment 2
[0068] Example 2: Calcein with a mass concentration of 0.2% labels the gastrointestinal tract of zebrafish at different temperatures
[0069] 1. Zebrafish sample pretreatment:
[0070] Use healthy and sexually mature AB zebrafish, put them into a mating tank at a ratio of 1:1 or 1:2, place a partition in the middle, and place them in a dark environment. Remove the partition before turning on the light the next day, and light stimulate Make it ovulate, fish out adult fish after ovulation half an hour, control the ovulation time within half an hour, to reduce the difference in development time between embryos. Collect the fertilized eggs and rinse them three times with new zebrafish embryo culture water to disinfect and clean the fertilized eggs; then transfer the fertilized eggs into clean water for zebrafish embryo culture, and control the temperature at 28°C with a 14h light / 10h dark cycle. Culture in light, change about 1 / 3 of the water every 24 hours, and suck out the dead...
Embodiment 3
[0081] Example 3: Calcein with a mass concentration of 0.2% labels the gastrointestinal tract of zebrafish at different times
[0082] 1. Obtaining and using zebrafish embryos
[0083] Use healthy and sexually mature AB zebrafish, put them into a mating tank at a ratio of 1:1 or 1:2, place a partition in the middle, and place them in a dark environment. Remove the partition before turning on the light the next day, and light stimulate Make it ovulate, fish out adult fish after ovulation half an hour, control the ovulation time within half an hour, to reduce the difference in development time between embryos. Collect the fertilized eggs and rinse them three times with new zebrafish embryo culture water to disinfect and clean the fertilized eggs; then transfer the fertilized eggs to clean water for zebrafish embryo culture, at 28°C, 14h light / 10h dark cycle under light control During cultivation, about 1 / 3 of the water was changed every 24 hours, and dead embryos were sucked ou...
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