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Sulfide ion detection kit based on bovine serum albumin-nano platinum/bismuth

A bovine serum albumin and detection kit technology, which is applied in the fields of analytical chemistry and nanometers, can solve the problems of complex sample pretreatment process, high cost, time-consuming processing process, etc. Effect

Inactive Publication Date: 2016-06-29
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require complex sample pretreatment processes, which are relatively time-consuming and expensive

Method used

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  • Sulfide ion detection kit based on bovine serum albumin-nano platinum/bismuth
  • Sulfide ion detection kit based on bovine serum albumin-nano platinum/bismuth
  • Sulfide ion detection kit based on bovine serum albumin-nano platinum/bismuth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Add 5 mL of 16 mmol / L chloroplatinic acid solution and 0.5 mL of 1.5 mol / L sodium hydroxide solution to 5 mL of bovine serum albumin aqueous solution with a concentration of 50 mg / mL, mix well, and react in a water bath at 80°C for 2 h. After the reaction, the solution was put into an ultrafiltration tube with a cut-off molecular weight of 3k, centrifuged and ultrafiltered at 6000r / min, and washed 3 times with water to obtain a bovine serum albumin-nano-platinum aqueous solution, and the bovine serum albumin-nano-platinum aqueous solution was freeze-dried to obtain bovine serum Albumin-nano platinum powder. Add 0.6 mL of bismuth nitrate solution with a concentration of 0.5 mmol / L and 1.8 mL of a phosphate buffer solution with a concentration of 10 mmol / L and pH=7 in 0.6 mL of bovine serum albumin-platinum aqueous solution with a concentration of 23.8 mg / mL, and mix After homogenization, react in a water bath at 80°C for 2.5 hours, put the reacted solution into an ultraf...

Embodiment 2

[0040] Add 1 mL of hydrogen peroxide with a concentration of 2 mol / L and 0.2 mL of 3,3',5,5'-tetra Methylbenzidine hydrochloride, 0.01mL bismuth-bovine serum albumin-platinum nano-platinum aqueous solution with a concentration of 4.76mg / mL, after mixing, incubate at 37°C for 10 minutes, and visually observe the color of the solution, as shown in figure 1 As shown, the solution is dark blue. Scanning with a UV-Vis spectrophotometer, such as figure 2 As shown, there is an absorption peak at 652nm.

Embodiment 3

[0042] Add 0.01 mL of bovine serum albumin-nano-platinum / bismuth solution with a concentration of 4.76 mg / mL to 0.4 mL of sulfide ion standard solution with a concentration of 40 μmol / L, place it at room temperature for 2 minutes after mixing, and add 1 mL of a concentration of 2mol / L of hydrogen peroxide, 0.2mL of 16mmol / L 3,3',5,5'-tetramethylbenzidine hydrochloride, 2.39mL of 4mmol / L ethylenediaminetetraacetic acid of 100mmol / L, pH=5 phosphate buffer solution, after mixing, incubate at 37°C for 10 minutes, visually observe the color of the solution, as image 3 Shown, solution color is significantly lighter than embodiment 2, scans with ultraviolet-visible spectrophotometer, as Figure 4 As shown, the absorbance of the solution is significantly less than that in Example 2, indicating that sulfur ions have a significant inhibitory effect on the catalytic activity of bovine serum albumin-nano-platinum / bismuth.

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Abstract

The invention discloses a sulfide ion detection kit based on bovine serum albumin-nano platinum / bismuth. Bovine serum albumin-nano platinum / bismuth reacts with sulfide ions and then simulates the change of peroxidase activity, 3,3',5,5'-tetramethyl benzidine hydrochloride is oxidized by hydrogen peroxide to develop a color under the catalysis action of bovine serum albumin-nano platinum / bismuth, and therefore changes of the solution color and ultraviolet absorption spectrum features are shown up. The linear range of absorbancy measurement is 0.08-4 micromol / L, and the detection limit is 50 nanomol / L. After a water sample is simply pretreated, the content of sulfide ions in the water sample can be measured well by adopting the method and the kit.

Description

technical field [0001] The invention relates to a rapid content determination method and a kit of sulfur ions using bovine serum albumin-nano platinum / bismuth as a simulated peroxidase, and belongs to the field of analytical chemistry and nanotechnology. Background technique [0002] With the rapid development of industrial technology, environmental problems have become more and more serious. Sulfide is a common chemical raw material and chemical pollutant in papermaking, petrochemical, leather and other industries, which is harmful to aquatic ecosystems and human health. Under acidic conditions, sulfide is transformed into hydrogen sulfide, which can harm cytochrome and oxidase, cause hypoxia in cells and tissues, and even endanger life. At the same time, hydrogen sulfide also corrodes metal equipment and pipelines, and can be oxidized into sulfuric acid by microorganisms, which intensifies corrosion. Sulfide is easily soluble in water. Therefore, sulfide is one of the im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/31
CPCG01N21/76G01N21/31
Inventor 陈伟沈奕珉何少斌邓豪华吴艳玉
Owner FUJIAN MEDICAL UNIV
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