Method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite

A technology of ascorbic acid and nitrite, applied in the direction of material electrochemical variables, can solve the problems of cumbersome sample processing process, and achieve the effect of miniaturization, wide linear range and high sensitivity

Active Publication Date: 2016-07-13
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
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  • Claims
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Problems solved by technology

In clinical applications, either different detection methods are used for different substances, or the sample processing process is cumbersome, or biological substances that are easily inactivated such as enzymes are used for labeling, which has a negative impact on the detection speed and effect.

Method used

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  • Method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite
  • Method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite

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Embodiment 1

[0028] (1) Preparation of working electrode

[0029] ①Configure Ni(NO) with a concentration of 0.6mol / L 3 ) 2 Ethanol solution, and drop-coated on the surface of tantalum wire with a diameter of 0.6mm, dried and placed on the sample stage of the DC arc plasma jet chemical vapor deposition system, and the height of the sample stage was adjusted so that it was about 1.5cm away from the plasma jet port. .

[0030] ②Close the reaction chamber, and evacuated the system. When the vacuum degree is below 1Pa, the flow rate is 2L / min of hydrogen, 1.5L / min of argon and 200mL / min of methane, and the reaction pressure is 100Pa~4000Pa, The arc voltage is 50V~70V, the arc current is 80A~130A, the substrate temperature is 500℃~1200℃, and after the growth time is 0.5min~30min, the graphene / tantalum wire sample is taken out as the working electrode. In the present invention, when the cavity pressure and pump pressure reach 3000Pa and 13000Pa, an arc current of 110A is applied, the temperatu...

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Abstract

The invention discloses a method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite.The method is implemented by a three-electrode system sensor with a working electrode, a reference electrode and a counter electrode.The working electrode is made of graphene/tantalum, graphene/boron-doped diamond films and graphene/titanium; the reference electrode can be a saturated calomel electrode or an Ag/AgCl electrode or a mercury/mercurous sulfate electrode; the counter electrode is a platinum sheet electrode.The method includes acquiring working curves by the aid of mixed solution of five to-be-detected materials with different concentrations and differential pulse voltammetry; diluting human serum; adjusting the concentrations until the concentrations are within the ranges of the working curves; comparing peak currents obtained by the aid of the differential pulse voltammetry to the working curves to obtain the concentrations of the five to-be-detected materials in the serum.The method has the advantages that characteristics of the concentrations of the five to-be-detected materials can be quickly simultaneously detected, and the method is high in sensitivity and low in detection limit.

Description

technical field [0001] The invention relates to an electrochemical method for simultaneously detecting five substances such as ascorbic acid, dopamine, uric acid, tryptophan and nitrite. Background technique [0002] Five substances, ascorbic acid, dopamine, uric acid, tryptophan and nitrite, play an important role in the metabolism of the human body. The content of the above substances contained in the body fluids of patients with liver function decline, food poisoning, etc., and play a preventive role. [0003] At present, redox titration, spectrophotometry, uricase-peroxidase coupling method, fluorescence spectrophotometry, colorimetry, etc. are commonly used in clinical medicine. In clinical applications, different detection methods are used for different substances, the sample processing process is cumbersome, or biological substances that are easily inactivated such as enzymes are used for labeling, which adversely affects the detection speed and effect. In addition,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/48
CPCG01N27/26G01N27/30G01N27/48
Inventor 李明吉高德兰李红姬李翠平曲长庆杨保和
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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