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Method for measuring Fo so as to estimate primary productivity of water body

A technology of primary productivity and water body, applied in the direction of testing water, material inspection products, etc.

Inactive Publication Date: 2016-07-13
INST OF AQUATIC LIFE ACAD SINICA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no literature or patent information to describe the use of modulating the chlorophyll fluorescence parameter Fo to characterize the content of chlorophyll a or the density of microalgae cells

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  • Method for measuring Fo so as to estimate primary productivity of water body
  • Method for measuring Fo so as to estimate primary productivity of water body
  • Method for measuring Fo so as to estimate primary productivity of water body

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Embodiment Construction

[0032] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0033] In the process of studying the chlorophyll fluorescence of microalgae, the inventor unexpectedly found that when the Fo value is 10 3 to 10 6 Within the range of , there is a significant linear relationship between Fo in modulating the chlorophyll fluorescence parameter and the content of chlorophyll a in the water and the number of algae cells. Based on this, in the laboratory culture process, Fo can be used as a parameter to characterize the algae cell density, chlorophyll a content, etc. of photosynthetic microalgae; The production activities such as bait feeding provide a basis; in water bodies prone to blooms, the outbreak of water blooms can be monitored by continuously measuring the sudden increase of...

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Abstract

The invention provides a method for measuring Fo to represent chlorophyll a content. The method comprises the following steps: establishing an Fo-chlorophyll a content coherency equation; measuring the Fo of a sample; substituting the Fo into the obtained equation, and calculating the chlorophyll a content. The invention further provides a method for estimating primary productivity of a water body, and the method comprises the step of substituting the chlorophyll a content obtained in the previous method into an equation of P=K.r.c (Chla).DH so as to obtain the primary productivity of the water body. The method further provides a method for measuring algae cell density, and the method comprises the following steps: establishing an Fo-algae cell density coherency equation; measuring the Fo of a sample; substituting the Fo into the obtained equation, and calculating the algae cell density. By adopting all the methods, chlorophyll content and algae cell density in a photosynthetic microalgae culture solution, an aquaculture water body or a natural water body can be easily measured, the primary productivity in the aquaculture water body or the natural water body can be easily estimated, the health level of the water body is assessed, and further a necessary basis is provided for aquaculture, environment monitoring and the like.

Description

technical field [0001] The present invention relates to the field of microalgae, and more particularly, the present invention relates to the use of Fo to measure the chlorophyll a content and algal cell density in a water body containing microalgae, and thereby estimate the primary productivity of the water body. Background technique [0002] Primary (or primitive) productivity, also known as water primary productivity, is the ability of photosynthetic plants (including bacteria) in water to fix the energy of sunlight to produce organic matter per unit area and unit time. Primary productivity is one of the important evaluation bases in the fields of aquaculture feeding amount and water environment nutrient type. In order to estimate the primary productivity, it is often necessary to measure the content of chlorophyll a, and then use the formula P=K r c(Chla) DH, where: P is the primary productivity, mg / (m 3 d); r is the assimilation coefficient; c(Chla) is the content of ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
CPCG01N33/18
Inventor 王强吴宗文谢伟陈辉何晨柳陈为先
Owner INST OF AQUATIC LIFE ACAD SINICA
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