A method for measuring primary productivity of water body based on pea fluorescence curve

A primary productivity and water body technology, applied in fluorescence/phosphorescence, measurement devices, material analysis through optical means, etc., can solve problems such as large errors

CN111024670BActive Publication Date: 2020-12-22HENAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-12-22

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Abstract

The invention provides a method for detecting primary productivity in a water body. The method comprises the following steps: S1, establishing an area-primary productivity correlation equation under aPEA fluorescence curve in the water body; S2, sampling a water body containing the same microalgae species as the water body, measuring and drawing a PEA fluorescence curve of the sample, and calculating the area under the PEA fluorescence curve; and S3, substituting the area under the PEA fluorescence curve measured in the S2 into the equation obtained in the S1 so as to calculate the primary productivity. By means of the invention, the chlorophyll content and the algae cell density in a photosynthetic microalgae culture solution, an aquaculture water body or a natural water body can be easily measured, the primary productivity in the aquaculture water body or the natural water body can be easily estimated, the health level of the water body can be easily evaluated, and thus necessary bases are provided for aquaculture, environmental monitoring and the like.
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Description

technical field

[0001] The invention relates to the field of microalgae, and more particularly, the invention relates to a method for measuring the chlorophyll-a content and the primary productivity of the water body in a water body containing microalgae based on a PEA fluorescence curve. Background technique

[0002] Primary productivity is the ability of photosynthetic plants (including bacteria) in water to fix the energy of sunlight to produce organic matter per unit area and unit time. In the aquaculture industry, it is necessary to evaluate the amount of feed and the scale of farming based on the primary productivity of the water body. The traditional method of estimating primary productivity needs to measure the content of chlorophyll a (Chla), and then estimate the primary productivity based on the content of Chla.

[0003] At present, the main methods for measuring the chlorophyll-a content of water body are colorimetric method and fluorescence method. The colorime...

Examples

Embodiment Construction

[0027] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0028] In the process of studying the fluorescence of microalgae chlorophyll, the inventor unexpectedly found that at a wavelength of 620nm, 1800μmol m -2 the s -1 Saturation pulse, 50 μmol m -2 the s -1 The area under the curve of the chlorophyll fluorescence dynamic induction curve (PEA fluorescence curve or OJIP curve) measured under the measurement light is linearly correlated with the content of chlorophyll a.

[0029] This article lists the relationship between the area under the PEA fluorescence curve and the content of chlorophyll a of several microalgae cultures by way of illustration. The microalgae listed include both prokaryotic cyanobacteria and eukaryotic green algae. PEA fluorescence curves can be s...