A biochip hybridization device

Abstract

The invention discloses a biological chip hybridizing device, characterized by comprising a hybridizing wet box and hybridizing boxes. The hybridizing wet box is internally and horizontally provided with a plurality of storage cavities for holding the hybridizing boxes, each hybridizing box includes a hybridizing box body and a hybridizing box cover, each hybridizing box body is internally provided with a chip holding cavity, the outer end surface of each hybridizing box cover is provided with a plurality of observation windows side by side, the edge of each observation window is provided with a sampling hole, the inner end surface of each hybridizing box cover is provided with a chip positioning block to fix a biological chip to a position, the position of one chip positioning block corresponds to that of the corresponding chip holding cavity, each hybridizing box body is connected with one end of the corresponding hybridizing box cover through hinges, and each hybridizing box body is connected with the other end of the corresponding hybridizing box cover through a lock assembly. The biological chip hybridizing device is simple in structure, chip sliding can be avoided effectively, hybridizing success rate is guaranteed, a constant hybridizing space is provided for hybridizing biological chips through the hybridizing wet box, other additional auxiliary devices are not required, and the cost is saved.

Description

technical field [0001] The invention relates to the field of biochip technology, in particular to a biochip hybridization device. Background technique [0002] With the rapid development of biotechnology and related disciplines, the application of biochips is becoming more and more extensive. The application of the biochip is to hybridize it with the labeled biological sample, and judge the quantity of the target molecule in the sample by detecting the strength of the hybridization signal. [0003] Chinese invention patent 200610114412.3 discloses a biochip hybridization box, including a base, an upper cover, a clamp groove and a bracket. When the hybridization box is used, the biochip is first placed on the bracket, and then the substrate is placed on the biochip On the surface, the biochip and the substrate are separated. When using a micropipette to add the sample to the reaction area, due to the small injection aperture, the tip of the conventional pipette can easily dr...

AI Technical Summary

Problems solved by technology

[0003] Chinese invention patent 200610114412.3 discloses a biochip hybridization box, including a base, an upper cover, a clamp groove and a bracket. When the hybridization box is used, the biochip is first placed on the bracket, and then the substrate is placed on the biochip On the surface, the biochip and the substrate are separated. When using a micropipette to add the sample to the reaction area, due to the small injection aperture, the tip of the conventional pipette can easily drive the substrate to shift, thus taking the substrate away. The surface of the biochip is prone to slide phenomenon, which leads to the failure of hybridization. In order to avoid the slide phenomenon, some operators will use the micropipette with the smallest tip to inject the reagent multiple times, which will cause air bubbles to enter reaction area, thereby increasing the failure probability of hybridization; in addition, after the biochip and substrate are installed, they need to be placed in an environment with a constant temperature such as constant temperature water, a constant temperature box or a hybridization furnace. At this time, the upper cover needs to be covered. The clamping groove seals and fixes the upper cover and the base, and then places the entire hybridization box in constant temperature water or other corresponding equipment, but the structure of the above-mentioned sealing and fixing method is complicated, and a certain extrusion force will be generated when the clamping groove is sealed and fixed , the samples and reagents in the reaction area of ​​the chip may be squeezed out of the reaction area by the extrusion force, thereby affecting the reaction of the biochip; at the same time, the hybridization box with the above structure occupies a large space and the production cost is high; In addition, constant temperature conditions generally need to be completed by additional instruments, which makes the experiment cost higher

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