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Method and markers for assessing the risk of having colorectal cancer

A colorectal cancer, risk technology, applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problems of low accuracy, imprecise results, and low accuracy

Inactive Publication Date: 2016-07-20
CHANG GUNG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, tomography is more prone to inaccurate results due to the problem of image resolution, and endoscopy is an invasive examination, which still has its risks. In terms of fecal occult blood examination, although it is cheap and easy to operate, it is not accurate. (accuracy) is not high
Fecal occult blood test with immunochemical method is currently available, which can avoid false negative and false positive errors caused by the patient's diet, but there is still considerable room for improvement in its accuracy
Therefore, in the current methods for assessing the risk of colorectal cancer, the assessment results are either low in accuracy or need to be checked in an invasive way. There is an urgent need for a novel method for assessing the risk of colorectal cancer in order to achieve reliable results. Detected in a non-invasive way and achieve high sensitivity

Method used

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  • Method and markers for assessing the risk of having colorectal cancer
  • Method and markers for assessing the risk of having colorectal cancer
  • Method and markers for assessing the risk of having colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0072] Experimental Example 1: This assessment method can be used to assess the risk of colorectal cancer in human individuals

[0073] Evaluation subjects and stool samples

[0074] In this experiment example, stool samples from 144 colorectal cancer patients and 390 healthy human individuals were collected from Chang Gung Memorial Hospital in Taiwan, and the expression levels of various microRNAs as shown in Table 1 were analyzed. Cancers were judged according to the 7th edition of the 2009 American Joint Committee on Cancer staging criteria (2009 American Joint Committee on Cancer staging criteria, 7th edition), and their clinicopathological factors were recorded, including age, sex and the results of the immune fecal occult blood test (iFOBT). For the collection of samples, the stool samples donated by colorectal cancer patients were collected. The stool samples were the remaining samples left after the conventional iFOBT test, and the patients had not received any treatme...

experiment example 2

[0091] Experimental Example 2: Comparison of the evaluation method with the immune fecal occult blood test

[0092] In Experimental Example 2, 390 healthy human individuals collected in Experimental Example 1 and 144 stool specimens diagnosed with colorectal cancer were collected, and according to the sampling method and expression measurement method of Experimental Example 1, the stool samples were detected The concentration (expression level) of the first microRNA and the second microRNA as listed in Table 1 in the specimen.

[0093] Then, according to Table 2, the combination ratio of the first microRNA and the second microRNA, and the individual represented by each stool sample source is a healthy control group or a patient with colorectal cancer to draw receiver operating characteristics with PASWStatistics18.0 software Curve (Receiveroperatingcharacteristiccurve, ROCcurve) and calculate the area under the receiver operating characteristic curve (AreaUndertheROCcurve, AUC...

experiment example 3

[0103] Experimental Example 3: Comparison of this evaluation method with the evaluation using a single microRNA

[0104] In Experimental Example 3, the experimental process and data calculation and arrangement can refer to the aforementioned Experimental Example 2. In this experimental example, compared to using the expression level of a single microRNA (the first microRNA or the second microRNA) to evaluate, using the first microRNA and the second microRNA shown in Table 2 The ratio of the expression amount of the nucleic acid will have a relatively good effect. For example, as shown in Table 7, miR-223 / miR-221, miR-223 / miR-222, miR-223 / miR-21, miR-223 / miR-93, miR-25 / miR-221 , miR-25 / miR-222, miR-25 / miR-21, miR-25 / miR-93, miR-25 / miR-141, miR-25 / miR-200c, and miR-25 / miR-191 The AUC value (diagnostic accuracy) was greater than the first microRNA (miR-223, miR-25) or the second microRNA (miR-221, miR-222, miR-21, miR-93, The AUC value (diagnostic accuracy) of miR-141, miR-200...

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Abstract

The disclosure discloses a method and markers for assessing the risk of having colorectal cancer. The disclosure discloses a method and markers for assessing the risk of having colorectal cancer for an individual by a stool sample. The method includes: detecting expression levels of a first microRNA and a second microRNA in the stool sample; and assessing the risk of having colorectal cancer for the individual based on a ratio between the expression levels of the first microRNA and the second microRNA. Here, the first microRNA is miR-223, miR-25, or miR-93, and the second microRNA is miR-221, miR-222, miR-21, miR-93, miR-141, miR-200c, miR-191, miR-17, miR-148a, miR-106a, miR-195, miR-20a, miR-181b, miR-145, miR-155, miR-106b, miR-24, miR-19b, miR-130b, or miR-18a. When the first microRNA is miR-93, the second microRNA is miR-17, miR-106a, miR-195, miR-20a, miR-181b, miR-155, miR-24, miR-19b, or miR-18a.

Description

technical field [0001] The present application relates to a method and a marker for assessing the risk of an individual suffering from colorectal cancer. Background technique [0002] Micro ribonucleic acid (micro-ribonucleic acid, microRNA), also known as miRNA, mi-RNA, microRNA or microRNA. MicroRNA mainly regulates gene expression in organisms by degrading messenger ribonucleic acid (mRNA) or inhibiting translation. It plays an important regulatory role in the growth and development of animals and plants, cell differentiation and apoptosis, and human diseases (such as tumors). The special function of microRNA is closely related to the pathogenesis of tumors, making it of great value in tumor classification and prediction. In addition to accurate tumor classification, the determination of miRNA can also grasp the individual differences of tumors, and then accurately and effectively use drugs. [0003] Colorectal cancer (CRC) is the top four deadly cancers. Around the w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 陈进勋张玉生詹雨昌陈嘉君张璧月
Owner CHANG GUNG UNIVERSITY