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Method for using a constant temperature total internal reflection microfluidic chip detection all-in-one machine

A technology of microfluidic chip and total internal reflection, which is applied in the direction of scattering characteristic measurement, measuring device, and material analysis through optical means, and can solve the problem of lack of using method of constant temperature all-in-one machine, hindering the development, manufacturing, processing and application of microfluidic technology and other problems, to achieve the effect of uniform and constant temperature, uniform and constant humidity

Active Publication Date: 2018-07-31
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

Due to the rapid development of microfluidic chips, the bottleneck hindering the development of microfluidic technology today is still the problems in manufacturing, processing and application that limited its development in the early days.
Total internal reflection optical detection technology is an optical detection technology that is compatible with microfluidic chip integration. However, when using these two technologies to detect living cells, there is a lack of a constant temperature all-in-one machine that meets the requirements of live cell detection and its use.

Method used

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  • Method for using a constant temperature total internal reflection microfluidic chip detection all-in-one machine
  • Method for using a constant temperature total internal reflection microfluidic chip detection all-in-one machine

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Embodiment Construction

[0022] see figure 1 , figure 2 , a constant temperature total internal reflection microfluidic chip detection all-in-one machine, which is composed of a main machine box 1 and a processing display 18, and is characterized in that: the airtight main machine box 1 has an opening on the vertical front side, and the inside of the box body passes The upper and lower box chamber partitions 5 divide the box interior into two layers, the lower floor box room 2 and the upper floor box room 13, and are respectively equipped with independent lower floor transparent doors 17 and upper floor transparent doors 14 at the opening of the box body; the lower floor box room 2 is installed There is an inverted fluorescent microscope 3, and the top of the inverted fluorescent microscope 3 lens is facing the observation window 4; the top of the upper and lower chamber partitions 5 above the observation window 4, that is, on one side of the upper and lower chamber partitions 5 in the upper chamber ...

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Abstract

The invention discloses a use method for a constant-temperature total-internal-reflection microfluidic-chip detection all-in-one machine. The use method comprises the steps: turning on a power supply of a host box body and a processing display; starting up a fan, a heating chamber heating device and an air purification device, loading a detection chip, adjusting a total-internal-reflection light source, a receiver and an inverted fluorescent microscope, carrying out detection, and carrying out shutdown. The use method has the advantages that a detection method using total internal reflection is provided and a detection method using the inverted fluorescent microscope is also provided so as to achieve multiple purposes and meet the requirements that different factors are simultaneously detected by one machine.

Description

technical field [0001] The invention relates to a method of using an in vitro diagnostic all-in-one machine, more specifically, a method for using a constant-temperature total internal reflection microfluidic chip detection all-in-one machine belonging to the field of in vitro diagnosis of medical equipment. Background technique [0002] In vitro diagnosis refers to the diagnosis of blood, body fluid, and tissue samples taken out of the human body for testing. At present, more than 80% of clinical disease diagnoses rely on IVD, which is known as the "eyes" of doctors. IVD plays an increasingly important role in modern society, especially pre-bed instant diagnosis plays an extremely important role in the whole process of disease prevention, diagnosis, monitoring and guiding treatment, and is indispensable for modern disease and health management Tool of. Due to the rapid development of microfluidic chips, the bottleneck hindering the development of microfluidic technology to...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N21/01
CPCG01N21/01G01N21/55G01N2021/0106G01N2021/555G01N2201/0231G01N2201/0233G01N2201/0238
Inventor 廖晓玲徐紫宸李波肖文谦
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY