Lung cancer screening kit
A kit and reagent technology, which is applied in the field of lung cancer screening kits and achieves good clinical application prospects.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Example 1 Relationship between FABP1 expression level and lung cancer
[0022] The experimental method is as follows:
[0023] 1. Clinical data
[0024] Fifteen patients with early lung cancer and 10 healthy controls were selected. The basic information is as follows:
[0025] Basic Information
lung cancer patients
number of people
15
10
age
61.2±12.2
49.5±10.6
male ratio
10(66.7%)
6(60.0%)
[0026] 2. Detection of FABP1 expression level (quantitative detection of FABP1 content)
[0027] Using the AAH-BLG-CUST kit purchased from RayBiotech (Catalog No.: AAH-BLG-CUST), the expression level of FABP1 was detected according to the following method:
[0028] 1. Sample dialysis
[0029] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb t...
Embodiment 2
[0057] Embodiment 2 Composition and method of use of the kit for detecting blood FABP1 expression level according to the present invention
[0058] 1. Composition of FABP1 Detection Kit
[0059] Detection kit (30 persons):
[0060]
[0061] 2. How to use the FABP1 detection kit
[0062] The method of use is as follows:
[0063] 1. Sample dialysis
[0064] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb the supernatant after the second centrifugation, namely plasma, as a test Specimens were stored at -80°C after aliquoting.
[0065] Plasma samples were dialyzed using dialysis tubing prior to biotin labeling. 100 μL of samples were taken respectively and added into dialysis tubes, and then dialyzed in 4000 mL of 1×PBS (pH=8) at 4° C. while stirring. The dialysate was changed every 3 hours, and the samples were collecte...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com