Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds

An immature, seed-based technology, applied in the field of preservation and propagation of extremely small populations, to achieve strong operability, save time for rapid propagation, and improve the level of reproduction

Active Publication Date: 2016-07-27
ZHEJIANG UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent need for a complete set of technologies that can improve the reproductive level of Vaccinium vaccinium to preserve Vaccinium custardum and prevent the extremely small population of Vaccinium vaccinia from becoming extinct due to the difficulty in breeding under natural conditions, but so far there is no report on this aspect

Method used

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  • Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds
  • Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds
  • Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds

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Embodiment 1

[0027] Materials: In this example, the immature seeds of Guangxi vaccinia collected in the wild in Fengyang Mountain Nature Reserve were used as explants for culture.

[0028] Step (1): Collect materials

[0029] The immature fruit of Guangxi bilberry was collected in Fengyang Mountain Nature Reserve from mid-to-late September to early October. The surface of the fruit is light green and the skin is hard.

[0030] Step (2): Material disinfection

[0031] The unripe fruits of Guangxi bilberry picked were first soaked and cleaned with detergent water (5ml detergent was added to 1L water) for 20 minutes, rinsed with tap water for 2 hours, and then operated on an ultra-clean workbench, using volume fraction Soak in 75% ethanol aqueous solution for 30 seconds, wash with sterile water 3 times for 1 minute each time, then sterilize with 0.1% mercuric chloride aqueous solution for 10 minutes, shake 3-5 times during this period, and rinse with sterile water 5 times , each time for 1 ...

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Abstract

The invention discloses a method for preserving minimum population Guangxi bilberry offspring by means of immature seeds.The problem that minimum population Guangxi bilberries in Zhejiang province can not be reproduced easily under the natural condition is solved.According to the method, immature fruits of the Guangxi bilberries are collected in a natural protecting area, after the surfaces of the fruits are sterilized, immature seeds and a part of ovary tissue are picked off, by means of the tissue cultivation technology, the Guangxi bilberry offspring is bred, and more Guangxi bilberry sterile seedlings are obtained within a short time.The method has the advantages that adopted explants are the immature seeds, an artificial culture medium is utilized for cultivating the explants, the defect that under the natural condition, seed propagation is not developed can be overcome, and more Guangxi bilberry plants can be obtained within a short term.The method is high in operability, simple, practical and low in pollution rate, and has great significance for preservation and propagation of minimum populations.

Description

technical field [0001] The invention relates to a method for preserving the offspring of a very small population of Guangxi vaccinia through immature seeds, and relates to the technical field of achieving the purpose of preserving and multiplying a very small population by improving the seed breeding level. Background technique [0002] Guangxi Vaccinium belongs to Rhododendronaceae Vaccimium (Vaccimium) plant, is an evergreen shrub, born in the forest at an altitude of 1200-1700 meters, on rocks in valleys or epiphytic on the trunk of Fagaceae. Guangxi bilberries are mainly distributed in Hunan, Guangdong and Guangxi. In 2003, the "Fengyang Mountain Rare Plants and Vegetation Survey" first discovered and reported the distribution of Guangxi bilberry in Zhejiang Province, but its quantity is rare, with less than 50 plants distributed in the whole province, and it was included in the list of extremely small population protection in Zhejiang Province . At present, the common...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
CPCA01G24/00A01G31/00A01H4/001A01H4/008
Inventor 陈利萍曹丽雯于宁宁李俊星叶立新刘胜龙彭辉刘玲娟
Owner ZHEJIANG UNIV
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