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A chamber for encapsulating secreting cells

A cell and cavity sealing technology, applied in the field of bioartificial organs, can solve problems such as tearing and less obvious

Active Publication Date: 2016-07-27
DEFYMED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to design organs that are large enough to achieve this, but then they have the disadvantage that after implantation they run the risk of tearing due to the movement of the patient (this problem is especially important for cells that only accommodate a limited number of cells). less obvious for micro-organs)

Method used

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  • A chamber for encapsulating secreting cells
  • A chamber for encapsulating secreting cells
  • A chamber for encapsulating secreting cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0161] Embodiment 1: the manufacture of semi-permeable membrane

[0162] Membranes were fabricated by preparing two porous PET (poly(ethylene terephthalate)) layers from a biocompatible PET film by a "track-etching" process, which was then combined with Density nonwoven PET layer (located between two porous biocompatible PET layers) laminated. Thermal lamination is performed without the use of adhesives. One of the porous PET layers has a 2.10 9 to 7.10 9 hole / cm 2 The pore density, and the internal pore diameter of 10 to 30nm. The film has a thickness of 8 to 12 μm. Other porous PET layers have 10 7 to 5.10 7 hole / cm 2 The pore density, and the internal pore diameter of 400 to 600nm. The film has a thickness of 30 to 50 μm. The total thickness of the film is 200 μm or less.

Embodiment 2

[0163] Embodiment 2: surface treatment of film

[0164] The membrane prepared according to Example 1 was subjected to a surface treatment according to the protocol of Example 1 of WO2012 / 017337.

[0165] The membrane was functionalized with a first layer of heparin mixed with ethylcellulose (EC) solution and then covered with a layer of hydroxypropylmethylcellulose (HPMC).

Embodiment 3

[0166] Example 3: Characterization of the permeability of the membrane

[0167]Glucose permeability, insulin permeability and immunoglobulin (IgG) permeability tests of previously prepared membranes were performed according to the following protocols:

[0168] Material

[0169] Diffusion chamber consisting of top and bottom compartments separated by a membrane (need to test for permeability) (tightness between the two compartments is provided by a seal), glucose (Fischer Scientific, Illkirch, France, number: G / 0500 / 53), NaCl, IgG (Sigma, Lyon, France, number: I9640), insulin (Sigma, number: I9278), distilled water.

[0170] Solution preparation

[0171] ·Normal saline

[0172] For 1 l: Dissolve 9 g of NaCl in 1 l of distilled water.

[0173] ·Glucose (4g / l)

[0174] For 1l: Dissolve 4g of glucose in 1l of normal saline.

[0175] IgG (5.75μg / ml)

[0176] For 60ml: Dilute 34.5ul IgG stock solution (10mg / ml) in 59.966ml saline.

[0177] Insulin (100μg / ml)

[0178] ...

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Abstract

The invention relates to an encapsulating chamber for secreting cells, comprising a closed shell made of a semi-permeable membrane, said membrane comprising at least one layer of porous biocompatible polymer, and one layer of non-woven biocompatible polymer.

Description

technical field [0001] The invention relates to the field of bioartificial organs which are implantable and which are in particular in the form of chambers for enclosing cells secreting substances of interest. A membrane enabling the encapsulation chamber and the bioartificial organ to be manufactured is also a subject of the present invention. Background technique [0002] The treatment of pathological conditions that require a continuous supply of therapeutically interesting substances to the body necessitates the development of devices that can be implanted in patients and that release these substances efficiently, sometimes over extended periods of time. [0003] To meet this need, bioartificial organs have been developed that house cells that produce one or more substances of therapeutic interest. The cells housed in the bioartificial organ are confined within an interior space or enclosed chamber, delimited by at least one semi-permeable membrane. When the membrane a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00C12N5/071A61K38/28A61K39/39C07K16/00
CPCA61K38/28A61K9/0024C12N5/0677A61K2035/128A61K35/39A61P3/10A61K9/5031A61K9/5047
Inventor R·布奥S·西格里斯特S·斯普罗
Owner DEFYMED
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